Polyclonal and monoclonal antibodies to Norwalk virus and method

Details Polyclonal and monoclonal antibodies to Norwalk virus and method Polyclonal and monoclonal antibodies to Norwalk virus and method

1993-02-04

2000-12-05

Stucker, Jeffrey

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Blood proteins or globulins, e.g., proteoglycans, platelet...

5303883, 435 7021, 436547, C07K 1600, C12P 2104, G01N 3353

Patent

active

06156883&

ABSTRACT:
Double-stranded cDNA was synthesized from nucleic acid extracted from Norwalk virus purified from stool specimens of volunteers. One clone was isolated from a cDNA library constructed in a pUC-13 vector after amplification of the cDNA. The specificity of this cDNA (pUCNV-953) was shown by hybridization assays. The cDNA reacted with post- (but not pre-) infection stool samples from Norwalk volunteers and with highly purified Norwalk virus, but not with other common enteric viruses such as hepatitis A virus and rotavirus. Finally, the probe detected virus in the same fractions of CsCl gradients in which viral antigen was detected using a specific Norwalk virus radioimmunoassay, and particles were detected by immune electron microscopy. Single-stranded RNA probes derived from the DNA clone after subcloning into an in vitro transcription vector were also used to show that the Norwalk virus contains a ssRNA genome of about 8 kb in size. The original clone was also used to detect additional cDNAs which represent at least 7 kb of nucleic acid of the Norwalk genome. The availability of a Norwalk-specific cDNA and the first partial genome sequence information allow rapid cloning of the entire genome and of establishment of sensitive diagnostic assays. Such assays can be based on detection of Norwalk virus nucleic acid or Norwalk viral antigen using polyclonal or monoclonal antibodies to proteins expressed from the cDNA or to synthetic peptides made based on the knowledge of the genome sequence. Vaccines made by recombinant DNA technology are now feasible.

REFERENCES:
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4751080 (1988-06-01), Wyatt et al.
patent: 4814268 (1989-03-01), Kreider et al.
Greenberg et al, J. Virol. 37:994-999 Mar. 1981.
Greenberg et al, J. Med Virol 2:97-108, 1978.
Treanor et al, Proc. Natl. Acad. Sci., USA, 85: 3613-3617 May 1988.
Walter, J. Immunol. Meth. 88: 149-161, 1986.
Dresser, Ch 8 of "Handbook of Experimental Immunochemistry" Weir et al, Blackwell Publ v. 1:8.1-8.21 1986.
Norrott, J. Med. Virology 44:280-286, 1994.
Ploegh, PNAS 77:6081-6085, 1980.
T. Tanaka et al.; High Yield Production of Monoclonal Antibodies to Recombinant Norwalk Virus-Like Particles by Oral Immunization; Presented at CDC meeting.
Bruce Alberts et al.: Molecular Biology of The Cell, Second Addition; Chapter 5; Basic Genetic Mechanisms, 258-266 (1989).
Chitra Mandal et al.; Production of Highly Specific Polyclonal and Monoclonal Antibodies Using Estradiol-3-O-Carboxymethyl Ether as Hapten; Steroids 52/5&6, 551-60, Nov. & Dec. 1988.
Ed Harlow et al.; Antibodies: A Laboratory Manual; 115, 141, 156-57, 242 (1988).
Bruce Alberts et al.; Molecular Biology of The Cell, Third Edition; The Functional Properties of Antibodies, pp. 1211-1227 (1994).
Eli Benjamini et al.; Immunology: A Short Course, Second Edition; pp. 174-175 (1991).
Evan M. Roitt et al.; Immunology; (1989).
Charles A. Janeway et al.; Immuno Biology: The Immune System in Health and Disease, Fourth Edition; pp. 99-100.
Xi Jiang et al.; Sequence and Genomic Organization of Norwalk Virus; Virology 195, 51-61 (1993).
Xi Jiang et al.; Norwalk Virus Genome Cloning and Characterization; Science, 250:1580-83.
Wang et al.; Sequence Diversity of Small, Round-Structured Viruses in the Norwalk Virus Group; Journal of Virology pp. 5982-5990 (1994).
Xi Jiang et al.; Detection of Norwalk Virus in Stool by Polymerase Chain Reaction; Journal of Clinical Microbiology pp. 2529-2534 (1992).
Appleton, H. "Small Round Viruses: Classification and Role in Food-Borne Infetions" in Novel Diarrhoea Viruses. (1987) pp. 108-125.
Caul, E.O., et al., J.Medical Virology 9:257-265 (1982).
Cubitt, W.D., et al., J. Infect.Diseases. 156(5):806-814 (1987).
Dingle, J.H., et al., Am. J. Hyg., 58:16-30 (1953).
Dolin, R., et al., Proc. Soc. Exp. Med. & Biol., 140:578-583 (1972).
Dolin, R., et al., J. Infect. Dis., 123:307-312 (1971).
Dupont, H.L., New England J. Med., 314(11):707-708 (1986).
Enzymology, vol. 70, 1980, pp. 104-142.
Geysen, et al., PNAS 8:3998-4002 (1984).
Gill, O.N., et al., Br. Med. J., 287:1532-1534 (1983).
Greenberg, H.B., et al., J. Med. Virol. 2(2):97-108 (1978).
Greenberg, H.B., et al., J. Virol. 37(3):994-999 (1981).
Gunn, R.A., et al., Am. J. Epidemiol., 115:348-351 (1982).
Hayaski, Y., et al., J. Clin. Microbiol., 27:1728-1733 (1989).
Jiang Xi, et al., J. Clin. Microbiol., 27:874-879 (1989).
Jiang Xi, et al., App. Environ. Microbiol., 53:2487-2495 (1987).
Jiang Xi, et al., App. Environ. Microbiol., 52:711-717 (1986).
Kapikian, et al., "Norwalk Group of Viruses" in Field's Virology, (2nd ed. 1990) pp. 671-693.
Kapikian, A.Z., et al., J. Virol., 10:1075-1081 (1972).
Kaplan, J., et al., Ann. Internal Med., 96(6):756-761 (1982).
Matsui, et al., J. Clin. Invest. 87:1456-1461 (1991).
Morse, D.L. et al., New Engl. J. Med., 314:678-681 (1986).
Murphy, A.N., et al., Med. J. Aust., 2:329-333 (1979).
Peptide Protein and Gene Technology, No. 6, Cambridge Research Biochemicals, Wilmington, Del.
Sekine, S., et al., Microbiol. Immunol., 33:207-217 (1989).
Thornhill, T.S., et al., J. Infect. Dis., 132:28-34 (1975).
Wilde, J., et al., J. Clin. Microbiol., 28(6):1300-1307 (1990).
Wilson, R., et al., Am. J. Public Health, 72:72-74 (1982).

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