Polycistronic expression vector construction

Details Polycistronic expression vector construction Polycistronic expression vector construction

1983-01-19

1987-12-15

Martinell, James

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 60, 435 70, 435 71, 435 91, 4351721, 4351723, 43524026, 435319, 435320, 536 27, 935 32, 935 34, 935 36, 935 70, C12N 500, C12N 1500, C12N 502, C12N 100, C12P 2100, C12P 2102, C12P 2104, C12P 1934, C07H 2104

Patent

active

047133392

ABSTRACT:
A polycistronic construction of xenogeneic sequences expressible in eukaryotic cells is described. DHFR encoding sequences under control of the same promoter with another foreign gene can be amplified with methotrexate, and thus cause coamplification of the foreign gene.

REFERENCES:
patent: 4399216 (1983-08-01), Axel et al.
Ringold et al, "Co-Expression and Amplification of Dihydrogolate Reductase cDNA and the Escherichia coli XGPRT Gene in Chinese Hamster Ovary Cells", J. Molec. Appl. Genet. 1:165, (1981).
Freifelder, D.: Molecular Biology--A Comprehensive Introduction to Prokaryotes and Eukaryotes, New York, Van Nostrand Reinhold Co., 1983, p. 481.
Alberts, B. et al: Molecular Biology of the Cell, New York, Garland Publishing, Inc., 1983, p. 332.
Lewin, B.: Genes, New York, John Wiley & Sons, 1983, p. 680.
O'Hare et al: "Transformation of Mouse Fibroblasts to Methotrexate by a Recombinant Plasmid Expressing a Prokaryotic Dihydrofolate Reductase", Proc. Natl. Acad. Sci., USA, 78:1527, (1981).
Moriarty et al, "Expression of the Hepatitis B Virus Surface Antigen Gene in Cell Culture by Using Simian Virus 40 Vector", Proc. Natl. Acad. Sci., USA, 78:2606, (1981).
Herman et al, "Polycistronic Vesicular Stomatitis Virus RNA Transcripts", Proc. Natl. Acad. Sci., USA, 77:4662, (1980).
Christman, Judith K. et al., Proc. Natl. Acad. Sci., 79:1815, (1982).
Kaufman, Randal J. et al., J. Mol. Biol., 159:601, (1982).
Ringold, Gordon et al., Journal of Molecular and Applied Genetics, 1:165, (1981).

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