Polycistronic expression vector construction

Details Polycistronic expression vector construction Polycistronic expression vector construction

1987-09-24

1990-10-23

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 693, 435 701, 435 703, 4351723, 435191, 4352401, 4352402, 435320, 935 32, 935 34, 935 36, 935 70, C12P 2100, C12N 1500, C12N 906, C12N 500

Patent

active

049651960

ABSTRACT:
A method for controlled production of a desired mature protein in a vertebrate host cell through the use of a polycistronic expression vector, which contains sequences coding for the desired protein and a secondary protein, is described. Both coding sequences are governed by the same promoter and are separated by translational stop and start signal codons. The expression of the secondary sequence effects control over the expression of the sequence coding for the desired protein, and the secondary protein functions as a marker for selection of transfected cells.

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