Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1989-05-23
1990-06-26
Niebling, John F.
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
204299R, G01N 2726
Patent
active
049369636
ABSTRACT:
The present invention provides for polycationic buffers for the gel electrophoresis of and methods for obtaining improved separation and resolution of nucleic acids. In particular, the invention provides for a buffer consisting essentially of histidine for the gel electrophoresis of oligonucleotides ranging from a few to about 70 nucleotides in length. Polycationic buffers may be used for both analytical and preparative purposes and result in a nearly two-fold improvement in both separation and resolution of the nucleic acid bands. Furthermore, the use of polycationic buffers reduces the electrophoresis time by a factor of about three due to the low conductivity of the buffers.
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Hayden Mark A.
Mandecki Wlodek
Abbott Laboratories
Hastreiter Roberta L.
Niebling John F.
Starsiak Jr. John S.
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