Polyamino acid synthetase and gene encoding the same

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...

Reexamination Certificate

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C435S252300, C435S252330, C435S254100, C435S320100, C536S023200

Reexamination Certificate

active

08030042

ABSTRACT:
The present invention provides an enzyme which catalyzes amino acid polymerization in the form of a non-ribosomal peptide synthetase (NRPS) and a gene encoding the same.

REFERENCES:
patent: 2003/0119018 (2003-06-01), Omura et al.
patent: 2005-52010 (2005-03-01), None
Kawai, T. et al.,Biosynthesis of ε-poly-L-lysine in a cell-free system of Streptomyces albulus, Biochemical and Biophysical Research Communications, vol. 311 (2003), pp. 635-640.
Saimura, M. et al.,Purification and Characterization of poly(ε-L-lysine)biosynthetic enzyme, Polymer Reprints, Japan, vol. 52, No. 5 (2003), p. 992 (with English translation).
Omura, S. et al.,Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites. Genbank, Oct. 11, 2001, Acc. No. AB070954, CDS: 6659-10534.NCBI Sequence Viewer v2.0.Web. May 7, 2008. <http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?15824207:DDBJ:5623295>.
Berg, H. et al.,Biosynthesis of the cyanobacterial reserve polymer multi-L-arginyl-poly-L-aspartic acid(cyanophycin),Eur. J. Biochem., vol. 267 (2000), pp. 5561-5570.
Aboulmagd, E. et al.,Purification of Synechocystis sp. Strain PCC6308 Cyanophycin Synthetase and Its Characterization with Respect to Substrate and Primer Specificity, Applied and Environmental Microbiology, vol. 67, No. 5 (May 2001), pp. 2176-2182.
Ashiuchi, M. et al.,Enzymatic Synthesis of High-Molecular-Mass Poly-γ-Glutamate and Regulation of Its Stereochemistry, Applied and Environmental Microbiology, vol. 70, No. 7 (Jul. 2004), pp. 4249-4255.
Shima, S. et al.,Poly-L-lysine Produced by Streptomyces. Part II. Taxonomy and Fermentation Studies, Agric. Biol. Chem., vol. 45, No. 11 (1981), pp. 2497-2502.
Supplementary European Search Report issued Dec. 27, 2010 in corresponding European Application No. EP 08 74 0665.
Database Uniprot [Online], retrieved from EBI accession No. A1TG57, Feb. 6, 2007.
Kazuya Yamanaka et al., ε-Poly-L-lysine dispersity is controlled by a highly unusual nonribosomal peptide synthetase, Nature Chemical Biology, vol. 4, No. 12, 2008, pp. 766-772.
Yoshimitsu Hamano et al., Development of gene delivery systems for the ε-Poly-L-lysine producer,Streptomyces albulus, Journal of Bioscience and Bioengineering, vol. 99, No. 6, 2005, pp. 636-641.
Y. Hamano et al., Biological function of thepldgene product that degrades ε-Poly-L-lysine inStreptomyces albulus, Applied Microbiology and Biotechnology, vol. 72, No. 1, 2006, pp. 173-181.
Nicholas Grammel et al., A β-lysine adenylating enzyme and a β-lysine binding protein involved in poly β-lysine chain assembly in nourseothricin synthesis inStreptomyces noursei, European Journal of Biochemistry, vol. 269, No. 1, 2002, pp. 347-357.

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