Polyamide resin-protide conjugate, preparation and uses

Details Polyamide resin-protide conjugate, preparation and uses Polyamide resin-protide conjugate, preparation and uses

1993-12-16

1996-04-30

Schofer, Joseph L.

Synthetic resins or natural rubbers -- part of the class 520 ser

Synthetic resins

Polymers from only ethylenic monomers or processes of...

4241931, 436518, 530334, 530402, C08F22602

Patent

active

055126480

ABSTRACT:
A large pore polyamide resin is useful for large peptide and protein (protide) synthesis. A method of preparing the same comprises mixing a dimethylacrylamide monomer with an unsaturated or alkenoyl amine monomer, a cross-linker and water, homogeneously emulsifying the aqueous mixture with an organic solvent in the presence of an emulsifier, adjusting the pH of the aqueous mixture during polymerization to 6-8.5 to produce large pore resin beads, and isolating the beads. The beads may be used as a solid phase substrate for the synthesis of a polyamide/protide conjugate. The polyamide resin/protide conjugate may be used, without separation of the protide from the resin or subsequent purification, for immunizing mammals, including humans, against the protide, for affinity purifying immunological molecules binding to the protide, and for immunoassays.

REFERENCES:
patent: 2576502 (1951-11-01), Dalton
patent: 2656339 (1953-10-01), Padbury
patent: 2893970 (1959-07-01), Caldwell et al.
patent: 3032539 (1962-05-01), Schuller et al.
patent: 3057833 (1962-10-01), Devlin
patent: 3061595 (1962-10-01), Dorion et al.
patent: 3062798 (1963-11-01), Lovett
patent: 3912607 (1975-10-01), Pierre et al.
patent: 3925267 (1975-12-01), Coupek et al.
patent: 4074039 (1978-02-01), Lim et al.
patent: 4138539 (1979-02-01), Landolt et al.
patent: 4172066 (1979-10-01), Zweigle et al.
patent: 4504640 (1985-03-01), Harada et al.
patent: 4528348 (1985-07-01), Turner et al.
patent: 4658000 (1987-04-01), Tyihak et al.
patent: 4812540 (1989-03-01), Kageno et al.
patent: 5028675 (1991-07-01), Kanda et al.
Atherton, E. et al., J. Amer. Chem. Soc. 97:6584 (1975).
Chersi, A. et al., J. of Immun. Methods, 122:285 (1989).
Fischer, P. et al., J. of Immun. Methods 118:119 (1989).
Takeyama, M. et al., Chem. Pharm. Bull., 37(3):834 (1989).
Gross, E. and Meienhofer, J., The Peptides: Analysis, Synthesis, Biology, 2:21 (1980).
Gutte, B. et al., J. of Bio. Chem. 246(6):1929 (1979).
Stahl, G. et al., J. Amer. Chem. Soc., 101:5383 (1979).

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