Polyamide bound protide, method of use thereof and kit

Details Polyamide bound protide, method of use thereof and kit Polyamide bound protide, method of use thereof and kit

1990-11-16

1992-01-28

Schofer, Joseph L.

Synthetic resins or natural rubbers -- part of the class 520 ser

Synthetic resins

Mixing of two or more solid polymers; mixing of solid...

525 5411, C08G 6348

Patent

active

050845098

ABSTRACT:
A polyamide resin for use in peptide and protein synthesis, and a method of preparing and using same. The polyamide resin is prepared by mixing a dimethylacrylamide monomer with an N-acrylyl-diaminoalkane functional monomer in an aqueous solution together with a cross-linker and emulsifying the aqueous solution in an organic solvent. An initiator and a promoter are added to polymerize the N-acrylyl-diaminoalkane functional monomer, dimethylacrylamide monomer, and cross-linker in the form of beads. The pH of the mixture is controlled during the polymerization. The beads are used as a solid phase for peptide and protein synthesis according to methods known in the art. The conjugate of the polyamide resin and the synthesized peptide or protein is used directly for immunoassays or immunization without the need for separation of the peptide or protein from the resin and subsequent purification.

REFERENCES:
patent: 2123013 (1983-06-01), Handa et al.
patent: 2134531 (1984-01-01), Furnuno et al.
patent: 2576502 (1951-11-01), Dalton
patent: 2893970 (1959-07-01), Caldwell et al.
patent: 3061595 (1962-10-01), Dorion et al.
patent: 3912607 (1975-10-01), Communal et al.
patent: 3925267 (1975-12-01), Coupek et al.
patent: 4074039 (1978-02-01), Lim et al.
patent: 4138539 (1979-02-01), Landolt et al.
patent: 4172066 (1979-10-01), Zweigle et al.
patent: 4528348 (1985-07-01), Turner et al.
patent: 4658000 (1987-04-01), Tyihak et al.
Prince, A. M., et al., 79 Proc. Natl. Acad. Sci. U.S.A. 579 (1982).
Sanchez, Y., et al., 18 Intervirology 209 (1982).
Arshady, R., et al., 1979 J.C.S. Chem. Comm. 425 (1979).
Dreesman, G. R., et al., 295 Nature 158 (1982).
Lerner, R. A., et al., 78 Proc. Natl. Acad. Sci. U.S.A. 3403 (1981).
Merrifield, R. B., "Solid-phase Peptide Synthesis," 32 Adv. Enzymology 221 (1969).
Atherton, E., et al., 97 J. Amer. Chem. Soc. 6584 (1975).
Stahl, G. L., et al., 101 J. Am. Chem. Soc. 5383 (1979).

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