Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1989-12-13
1994-05-31
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 792, 436518, 436530, 436 87, 436804, G01N 3353, G01N 33543
Patent
active
053169123
ABSTRACT:
A method for detecting a glycoprotein using a solid support is disclosed where the glycoprotein is oxidized by periodate, polyacrylic polyhydrazide which is a copolymer having repeating units possessing a hydrazide group and repeating units possessing hydroxyl groups is coupled to the oxidized glycoprotein and a glycoenzyme or radioactive compound containing aldehyde groups or activated ketone groups is coupled to the polyacrylic polyhydrazide which allows for detection of the glycoprotein. The glycoprotein may be directly attached to the solid support or may be bound to an antigen which is immobilized on the solid support.
REFERENCES:
patent: 4778751 (1988-10-01), El Shami et al.
Kozulic et al "Preparation of the stabilized glycoenzymes by cross-linking their carbohydrate chains" Chem. Abstracts 109(25):Abstract No 225585p (Dec. 1988.
Bayer et al "Enzyme-based detection of glycoproteins on blot transfers using avidin-biotin technology" Anal. Biochem. 161 pp. 123-131 (1987).
Kozulic et al. "Poly-N-acryloyl-Tris gels as anticonnection media for electrophoresis and isoelectric focusing" Anal. Biochem. 163 pp. 506-512 (1987).
Kozulic et al. "Electrophoresis of DNA restriction fragments in poly-N-acryloyl-Tris gels" Anal Biochem. 170 pp. 478-484 (May 1, 1988).
Heimgartner et al, "Polyacrylic Polyhydrazides as Reagents for Detection of Glycoproteins", Analytical Biochemistry, 181, 1989, pp. 182-189.
Heimgartner et al, "Polyacrylic polyhydrazides as novel reagents for detection of antibodies in immunoblotting assays", Journal of Immunological Methods, 132, 1990, pp. 239-245.
Miron et al, "Polyacrylhydrazido-Agarose: Preparation Via Periodate Oxidation and Use For Enzyme Immobilization and Affinity Chromatography", Journal of Chromatography, 215, 1981, pp. 55-63.
Bartles et al, "I-Wheat Germ Agglutinin Blotting: Increased Sensitivity with Polyvinylpyrrolidone Quenching and Periodate Oxidation/Reductive Phenylamination" Analytical Biochemistry, 140, 1984, pp. 284-292.
Heimgartner Urs
Kozulic Branko
Mosbach Klaus
Bidwell Carol E.
Farley Walter C.
Kepplinger Esther L.
Orzechowski K. L.
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