Polyacrylamide gels with improved detection of protein

Details Polyacrylamide gels with improved detection of protein Polyacrylamide gels with improved detection of protein

1991-11-07

1994-02-01

Housel, James C.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

436 94, 436161, 436178, 2041826, 2041828, G01N 3000, G01N 3368

Patent

active

052831965

ABSTRACT:
Amine-acryloyl and -methacryloyl derivatives and 1,6-heptadiene-4-ol can be used to provide crosslinked polyacrylamide gels which are useful in protein and nucleic acid separation and detection. The gels crosslinked with the compounds of the present invention exhibit much less background silver staining than previously known acrylamide gels.

REFERENCES:
patent: 4189370 (1980-02-01), Boschetti
patent: 4421915 (1983-12-01), Ponticello et al.
patent: 4535010 (1985-08-01), Axen et al.
patent: 4555490 (1985-11-01), Merril
patent: 4654132 (1987-03-01), Takagi et al.
patent: 4874490 (1989-10-01), Hochstrasser
patent: 4892814 (1990-01-01), Harrington et al.
Artoni et al., Analytical Biochemistry 137: 420-428, 1984.

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