Polyacrylamide gel for electrophoresis, polyacrylamide gel...

Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...

Reexamination Certificate

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C204S469000, C204S606000, C546S261000, C546S262000, C546S263000, C546S264000, C546S265000, C546S267000, C546S027000, C524S555000, C526S303100

Reexamination Certificate

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07901559

ABSTRACT:
It is intended to provide a method for easily detecting a phosphorylated peptide (protein) in a test sample by using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) which has been conventionally employed in analyzing a protein; a polyacrylamide gel for electrophoresis to be used in the method; a method of producing the gel; and a synthesizing intermediate in producing the gel. The polyacrylamide gel for electrophoresis to be used in the method of the invention is characterized in that at least a part of the structure thereof has a structure represented by the following formula (I); wherein M2+represents a transition metal ion; and X represents a linker group.

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Eiji Konoshita et al.—“Phosphase-binding Tag, a New Tool to Visualize Phosphorylated Proteins”—Molecular & Cellular Proteomics, vol. 5, No. 4, Dec. 11, 2005.

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