Pollen tube growth assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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436519, 436548, 436811, G01N 3300, G01N 33554

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active

048973459

ABSTRACT:
This invention provides an immunologic method for measuring plant pollen germination and pollen tube growth. This method is based on the finding that an antibody having specificity to .beta.-L-arabinofuranosyl residues binds to the surface of in vitro grown pollen tubes. Antibody binding can be measured qualitatively or quantitatively using a calibration curve. The immunoassay is useful for screening plants for response to substances that affect plant germination or pollen tube growth.

REFERENCES:
patent: 4528267 (1985-07-01), Calenoff et al.
Pettitt, J. M., "Ultrastructural . . . Pollen Tube Wall . . .", Chemical Abstracts 98, p. 404, #31522z, Jan. 31, 1983.
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Feder, W. A. (1986) in Biotechnology and Ecology of Pollen, (D. L. Mulcahy, G. D. Mulcahy, E. Ottaviano (eds.), Springer-Verlag, New York, pp. 89-94.
Searcy, K. B. and Mulcahy, D. L. (1985) Amer. J. Bot. 72:1700-1706.
Searcy, K. B. & Mulcahy, D. L. (1986 ) in Biotechnology and Ecology of Pollen, (D. L. Mulcahy, G. D. Mulcahy, E. Ottaviano (eds.) Springer-Verlag, N.Y., pp. 159-164.
Shivanna, K. R. et al. (1981) Protoplasma 107:319-337.
Dickinson, H. G. et al. (1982), Proc. R. Soc. B 215:45-62.
Williams, E. G. et al, (1982), Planta 156:517-519.
Harris, P. J. et al, (1984), Oxford Surveys Plant Molec. Cell Biol. 1:161-203.
Rae, A. L. et al. (1985), Planta 166:128-133.
Anderson, M. A. et al. (1984), Plant Physiol. 75:1013-1016.

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