Pollen-mediated gene transformation in plants

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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4353171, 4353201, 4351721, 935 59, 935 64, C12N 1500, A01H 100, A01H 102

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active

050495003

ABSTRACT:
A novel method for efficiently carrying out pollen-mediated gene transformation of flowering plants is described. Utilizing novel DNA constructs incorporating exogenous DNA fragments coding for specific enzymes, the method can product transformed plants exhibiting novel, useful traits. Confirming hybridization tests have been carried out to identify the exogenous DNA transformed into the plant.

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Ohta (1986) Proc. Nat. Acad. Sci., vol. 83, pp. 715-719.
Krens et al. (1984) in IK Vasil, ed., Cell Culture and Somatic Cell Genetics of Plants, vol. 1, Academic Press, N.Y., pp. 522-534.
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Spielman and Simpson, 1986, Mol. Gen. Genet. 205:34-41.
Lindsey and Jones, 1989, Plant Cell Reports 8:71-74.
Moyne et al., 1989, Plant Cell Reports 8:97-100.

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