Polarized light microscopy

Details Polarized light microscopy Polarized light microscopy

1995-02-17

1996-09-24

Henry, Jon W.

Optical: systems and elements

Compound lens system

Microscope

359386, 359494, 359499, G02B 2114

Patent

active

055596304

ABSTRACT:
In the establishment of interference colors in weakly birefringent materials within living organisms, in fixed tissues or in preparations of liquid crystals, a compensating birefringent plate is positioned in series with the object in the direction of the light path between a crossed polarizer and analyzer in a polarizing microscope, and is aligned with the vibrational direction of its slow wave at a small angle of horizontal rotation from the vibrational direction of the polarizer or of the analyzer. The small angle of rotation is between 2.degree. and 15.degree., more especially 4.degree. and 7.5.degree.. The chromatic response (color intensity) is enhanced and increased color contrast is obtained for all live organisms, freshly fixed sections, and liquid crystal preparations.

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patent: 4634240 (1987-01-01), Suzuki et al.
patent: 4795246 (1989-01-01), Lord
patent: 4873653 (1989-10-01), Grosskopf
patent: 5303082 (1994-04-01), Obuchi et al.

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