Polarized light microscopy

Optics: measuring and testing – By polarized light examination – With polariscopes

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G01N 2121

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active

055217052

ABSTRACT:
A method for determining polarization properties of light from an object uses a detector for detecting the intensity of light incident thereon. A pair of variable retarders are positioned in the optical path with their slow optical axes at a 45.degree. angle to each other, and a polarized light analyzer is also placed in the optical path between the light retarders and the detector. For each of the light retarders, a base retardance level is determined. The base retardance level is such that when each of the light retarders is set at the base retardance thereof and light from an object is circularly polarized, the light analyzer will cause substantial extinction of light from the object that traverses the analyzer and the intensity of light from the object incident on the detector will be substantially zero. Then, the retardance of the light retarders is set at a first retardance level in which the retardance of at least one of said retarders is different from the base retardance thereof and the intensity of light incident on said detector when said light retarders are set at the first retardance level is determined. This procedure is repeated with the retardance of the light retarders set at a second, and different retardance level. The polarization properties of the light is determined from the intensities measured at the different retardance levels.

REFERENCES:
patent: 3741661 (1973-06-01), Yamamoto et al.
patent: 3992104 (1976-11-01), Watanabe
patent: 4523848 (1985-06-01), Gorman et al.
patent: 4796995 (1989-01-01), Salzman et al.

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