(+)-Cycloolivil as antioxidant obtained from natural...

Drug – bio-affecting and body treating compositions – Plant material or plant extract of undetermined constitution... – Containing or obtained from a tree having matured height of...

Reexamination Certificate

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Reexamination Certificate




This invention relates to the isolation of compound namely (+)-Cycloolivil from a new plant source,
Stereospermum Personatum
in good yield. It is proved to be useful and better antioxidant molecule than the presently used medicinally important lipophilic antioxidants namely Prubucol and &agr;-Tocopherol. It may have better therapeutic potential in inflammatory disease conditions, atherosclerosis, diabetic complications, cancer, hepatotoxicity and variety of disease conditions mediated through or fostered by oxidative stress and/or overt oxidative burden due to increased generation or under scavenging of free radicals.
There is a considerable amount of epidemiological evidence indicating association between diet rich in fruits and vegetables and a decreased risk of cardiovascular disease and certain forms of cancer. It is generally assumed that the active principles contributing to these protective effects are nothing but primarily, the antioxidant phytochemicals.
Research in the past decades have accumulated enough evidence to show the beneficial effect of free-radical scavengers/antioxidants as antimutagenic, anti-inflammatory, antiamerosclerotic, antidiabetic, antihepatotoxic, antiageing and in a variety of neurological disorders. The search for new antioxidant principles is becoming therefore, essential to improve the pharmacological treatment of pathological conditions such as cataract, rheumatic diseases, atherosclerosis, Alzheimer's disease and other neurodegenarative conditions.
The pharmacological approaches therefore have focused on the search of potential resources rich in with antioxidant principles. The medicinal importance of plants bearing rich proportion of antioxidant principles is therefore becoming hot item.
Stereospermum personatum
is a medicinal plant used in traditional Indian System of Medicine and is widely advocated in the preparations for diuretic, lithontriptic, expectorant, cardiotonic, aphrodisiac, appetizer, anti-inflammatory, antibacterial, dyspepsia, diarrhea, renal and vesical calculi, cough, asthma, hyperdipsia, hemorrhoids and hyperacidity disease conditions (Indian Medicinal Plants, Vol. 5 p 192). It is further reported to possess antibacterial, antifungal, hypoglycemic activity, and against p388 lymphocytic leukemic cells (Ind. Jour, of Exp. Biol., 1971,9, 100). Hence, it becomes pertinent to look for the molecules possessing such important biological properties. In this connection, the phytochemical investigation of
Stereospermum personatum
has been taken up. The applicants efforts led to the isolation of (+)-Cycloolivil in good yield (+)-Cycloolivil is known to possess several activities, which are shown in Table-1
Cyclic AMP
Chem. Pharm. Bull, 1981,
29, 3586-92
Antihypertensive and
Chem. Pharm. Bull, 1986,
antagonist activity
34, 3514-17
Binding to human sex
Plant. Med. 1997, 63,
The main object of the invention is to provide a novel composition containing (+)-Cycloolivil and an additives, which is used as an antioxidant.
Another object is to provide a new source of obtaining (+)-Cycloolivil in significant yield.
Yet another object of the invention is to provide a process for producing (+)-Cycloolivil from a plant source namely
Stereospermum personatum.
Yet another object of the invention is relates to use of (+)-Cycloolivil as an antioxidant.
Accordingly, the invention provides a novel composition containing (+)-Cycloolivil and useful as antioxidant. The invention further provides a method for the isolation of (+)-Cycloolivil from new sources namely
Stereospermum Personatum.
In accordance with this invention, it has been found that (+)-Cycloolivil is isolated from a new source,
Stereospermum Personatum
in significant yield. In addition, it has been found that (+)-Cycloolivil shows antioxidant property.
Antioxidant compounds recently have attracted the attention due to their broad spectrum of activities in disorders of multiple origin viz., coronary heart disease, cancer, diabetes, rheumatic disorders and inflammatory conditions where free radicals play important role. Much attention is being directed now to harness and harvest the antioxidant compounds from natural resources.
The compound (+)-Cycloolivil is used in pure form. Hence, the usage may be more advantageous than a mixture of compounds having similar properties, which are in current use. It is also important to note that the process of isolation of (+)-Cycloolivil is highly economical.
Accordingly, the present invention provides a useful source,
Stereospermum Personatum,
comprising antioxidant principles of which (+)-Cycloolivil is isolated as a pure and potent antioxidant molecule. (+)-Cycloolivil has been compared with existing pharmacologically/therapeutically accepted antioxidant Probucol and alpha-tocopherol. It is found that (+)-Cycloolivil is better than the above mentioned reference drugs and hence may be used with pharmaceutically/therapeutically acceptable additives.
In an embodiment of the present invention, (+)-Cycloolivil may be effective at a less amount than the reference drugs mentioned above.
One embodiment of the present invention relates to a pharmaceutical composition useful as antioxidant, said composition comprising an effective amount of (+)-Cycloolivil in combination with or associated with a pharmaceutically acceptable additives.
In another embodiment of the invention, the pharmaceutically acceptable additive is selected in such a manner that it does not affect or interfere with the efficacy of (+)-Cycloolivil.
In still another embodiment of the invention, the additive is selected from nutrients such as carbohydrates, sugar, proteins, fats and pharmaceutically acceptable carrier.
In an embodiment of the present invention, the ratio of (+)-Cycloolivil to the additive is in the range between 0.1:10 to 3:10, preferably 0.4:10 to 2:10.
In yet another embodiment of the invention, the amount of (+)-Cycloolivil administered is in the range between 300 mg to 380 mg per dose at least twice a day.
In yet another embodiment of the invention, the composition is administered through oral route in the form of tablets, capsules, syrup or powder.
In one, more embodiment of the invention relates to process for isolation of (+)-Cycloolivil from the plant
Stereospermum personatum
said process comprising the steps of:
a) extracting the dried wood powder of
Stereospermum Personatum
with hexane;
b) further extracting the residue from step (a) with chloroform;
c) concentrating the chloroform solution from step (b) under vacuum;
d) absorbing the dark brown extract on a silicagel (60-120 mesh) and loaded on silicagel (60-120 mesh) column (4 cm dia to height of 100 cm);
e) eluting the column with chloroform methanol gradient, and
f) collecting the eluted fraction with 5% methanol in chloroform and concentrating the fraction to obtain pure (+)-Cycloolivil.
In another embodiment of the present invention, the solvents used are selected from hexane, chloroform and methanol.
In another embodiment of the present invention, the yield of (+)-Cycloolivil obtained is about 0.061% of the plant dried material.
One more embodiment relates to use of (+)-Cycloolivil as an antioxidant or free-radical scavenger animals and human beings.
In yet another embodiment relates to use of (+)-Cycloolivil for the manufacturing of a composition useful as antioxidant.
In yet another embodiment of the present invention, (+)-Cycloolivil is administered orally.
Yet another embodiment of the invention relates to a method for the treatment of free-radical scavenging, comprising the steps of administration of an effective amount of (+)-Cycloolivil to a subject in need thereof, preferably, (&p


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