Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-06-06
1998-09-15
Ziska, Suzanne E.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 721, 4352401, 4351723, 424 9321, G01N 3353, G01N 33567, C12N 500, C12N 1500
Patent
active
058076867
ABSTRACT:
A cell population which is composed of cells bearing the stem cell marker CD34 and which are small in size and have little granulation are obtained by separating low density mononuclear hematopoietic cells according to size and then selecting for CD34.sup.+ cells in the smallest size fraction. The size of the cell population corresponds to that obtained at a flow rate of 25-29 ml/min in a counterflow elutriation method using a rotor equivalent to Beckman JE 5.0 spun at 900.times.g. This population of cells consists essentially of very early progenitor cells and the cells are useful in autologous bone marrow transplantation as well as gene therapy.
REFERENCES:
patent: 4489710 (1984-12-01), Spitler
patent: 5061620 (1991-10-01), Tsukamoto
Goldstein, in The Lectis, Chapter 2, p. 155, Edited by Liener, Sharon and Goldstein, 1986.
Visser et al J Exp. Med. 59: 1576, 1984.
Jones et al. Nature 347: 188, 1990.
Boggs et al. Int. J. Cell Cloning 8: 80, 1990.
Brandt et al J. Clinical Invest 82: 1017, 1988.
Andrews et al J Exp. Med 172: 355, 1990.
Visser et al., "Isolation of murine pluripotent hemopoietic stem cells" J. Exp. Med. (1984) 59:1576-1590.
Jones et al., "Separation of pluripotent haematopoietic stem cells form spleen colony-forming cells" Nature (1990) 347:188-189.
Wagner et al., "Separation of primitive human hematopoietic stem cells from committed progenitors by counterflow elutriation (CE) followed by a soybean agglutinin negative depletion and CD34 positive selection" Blood (1991) 78:403a (abstract No. 1603).
Boggs et al Int J. Cell Cloning 8: 80, 1990.
Wagner et al (Abstract D211), J. Cell. Biochem Supp 16 A, 1992.
Brandt et al J Clin Invest 82: 1017, 1988.
Jones et al Nature 347: 188, 1990.
Wagner et al Blood 78: 403a, 1991 (Abst 1603).
Lebkowski Jane S.
Wagner John E.
Regents of University of Minnesota
Rhone-Poulenc Rorer Pharmaceuticals
Ziska Suzanne E.
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