Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Patent
1997-02-27
2000-08-15
Crouch, Deborah
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
435213, 435378, 435405, 435406, 435408, C12N 500, C12N 502, C12N 966
Patent
active
061035238
ABSTRACT:
The present invention relates to a rabbit embryonic stem (ES) cell line, comprising at least 70%, preferably 80 to 90% undifferentiated cells and obtainable by isolating the inner cell mass of 5.5 days postcoitus blastocysts and culturing them on feeder cells in rabbit ES medium. The invention further relates to further optimization of derivation and maintainance of the cell line and to the use thereof in inter alia generation of chimeric rabbits.
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Wurst et al in Gene Targeting: A Practical Approach, A. L. Joyner, ed., IRL Press, Oxford university Press, Oxford, England, 1993, pp. 33-61.
Clark et al in Transgenic Animals, F. Grosveld and G. Kollais, eds., Academic Press, London, England, 1992, pp. 247-270.
"Derivation and characterization of putative pluripotential embryonic stem cells from preimplantation rabbit embryos," Graves et al., Molecular Reproduction and Development, vol. 6, pp. 424-433.
"Pluripotency of cultured rabbit inner cell mass cells detected by isozyme analysis and eye pigmentation of fetuses following injection into blastocysts or Morulae,".
Giles et al., Molecular Reproduction and Development, vol. 36, pp. 130-138 (1993).
"Nuclear transfer of putative rabbit emvryonic stem cells leads to normal blastocyst development," Du et al., Journal of Reproduction and Fertility, vol. 104. pp. 219-223 (1995).
"A method for cultivating morphologicalcy undifferentiated embryonic stem cells from porcine blastocytes," Strojek et al., Theriogenology, vol. 33, No. 4, pp. 901-913 (1990).
"Dispersing and Disruption of Tissue," Bashor, Methods in Enzymology, Academic Press, vol. LVIII, pp. 119-131 (1979).
Moreadith Randall
Schoonjans Luc
Crouch Deborah
Thromb-X N.V.
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