Measuring and testing – Liquid analysis or analysis of the suspension of solids in a... – Content or effect of a constituent of a liquid mixture
Reexamination Certificate
2008-11-13
2011-12-13
Williams, Hezron E (Department: 2856)
Measuring and testing
Liquid analysis or analysis of the suspension of solids in a...
Content or effect of a constituent of a liquid mixture
Reexamination Certificate
active
08074494
ABSTRACT:
The invention provided is a split-free nano-liter flow liquid chromatography system comprising at least a first and a second chromatographic column connected in series, wherein an autosampler is provided and programmed to inject samples and eluent solutions of increasing eluent concentration for gradually eluting analytes from the first column into the second column (without adversely affecting separation in the second column), said solutions segmented from the mobile phase of the liquid chromatography system by air plugs.
REFERENCES:
patent: 6613235 (2003-09-01), Anderson et al.
patent: 2006/0157647 (2006-07-01), Siuzdak et al.
patent: WO 2004/017040 (2004-02-01), None
Gu et al., “Capillary array reversed-phase liquid chromatography-based multidimensional separation system coupled with MALDI-TOF-TOF—MS detection for high-throughput proteome analysis,”Journal of Proteome Research(2006) 5: 3186-3196.
Andersen Michael Barrett
Sørensen Ole Tang
Theodorsen Søren
Vorm Ole
Frank Rodney T
Merchant & Gould P.C.
Proxeon Biosystems A/S
Williams Hezron E
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