pLS010 plasmid vector

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435320, 935 29, 935 22, C12N 1500, C12N 100

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active

047299541

ABSTRACT:
Disclosed is recombinant plasmid pLS101, consisting essentially of a 2.0 Kb malM gene fragment ligated to a 4.4 Kb T.sub.c r DNA fragment, which is particularly useful for transforming Gram-positive bacteria. This plasmid contains at least four restriction sites suitable for inserting exogeneous gene sequences. Also disclosed is a method for plasmid isolation by penicillin selection, as well as processes for enrichment of recombinant plasmids in Gram-positive bacterial systems.

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patent: 4393137 (1983-07-01), Manis et al.
Stassi et al., Proc. Natl. Acad. Sci., 78:7028-7032 (1981).
Stassi et al., Genetics Exchange, edited by Stoeiks et al., Marcel Dekker, Inc. pp. 235-246 (1982).
Locks et al., Cell 31:327-336 (1982).
Locks, Genetics 53:207-235 (1966).
Lopez et al., J. Bacterial, 150:692-701 (1982).
Ghei et al., J. Bacterial, 93:816-829 (1967).
Marcina et al., J. Bacterial, 143:1425-1435 (1980).
Currier et al., Aval. Biochem. 76:431-441 1976.

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