Image analysis – Applications – Biomedical applications
Reexamination Certificate
2007-05-15
2007-05-15
Wu, Jingge (Department: 2624)
Image analysis
Applications
Biomedical applications
C435S006120
Reexamination Certificate
active
11097451
ABSTRACT:
Image analysis methods and apparatus are used for determination of the ploidy of cells. The methods may involve segmenting an image to identify one or more discrete regions occupied by cells or nuclei, determining the presence of a particular ploidy indicator feature within the region(s), and providing a value of the indicator feature to a model that classifies cells' ploidy on the basis of the indicator feature. In some embodiments, the indicator feature is a level of DNA in a cell. In certain embodiments, the method further comprises treating one or more cells with a marker that highlights the ploidy indicator feature. In certain embodiments, the cells are treated prior to producing one or more images of the one or more cells. In certain embodiments, the ploidy indicator feature comprises DNA and the marker co-locates with DNA and provides a signal that is captured in the image. In certain embodiments, the signal comprises a fluorescent emission.
REFERENCES:
patent: 4818710 (1989-04-01), Sutherland et al.
patent: 4922092 (1990-05-01), Rushbrooke et al.
patent: 4959301 (1990-09-01), Weaver et al.
patent: 4965725 (1990-10-01), Rutenberg
patent: 5016283 (1991-05-01), Bacus et al.
patent: RE34214 (1993-04-01), Carlsson et al.
patent: 5281517 (1994-01-01), Bacus et al.
patent: 5287272 (1994-02-01), Rutenberg et al.
patent: 5326691 (1994-07-01), Hozier
patent: 5355215 (1994-10-01), Schroeder et al.
patent: 5526258 (1996-06-01), Bacus
patent: 5548661 (1996-08-01), Price et al.
patent: 5655028 (1997-08-01), Soll et al.
patent: 5710022 (1998-01-01), Zhu et al.
patent: 5733721 (1998-03-01), Hemstreet et al.
patent: 5741648 (1998-04-01), Hemstreet et al.
patent: 5768412 (1998-06-01), Mitsuyama et al.
patent: 5776748 (1998-07-01), Singhvi et al.
patent: 5777888 (1998-07-01), Rine et al.
patent: 5790692 (1998-08-01), Price et al.
patent: 5790710 (1998-08-01), Price et al.
patent: 5804436 (1998-09-01), Okun et al.
patent: 5856665 (1999-01-01), Price et al.
patent: 5893095 (1999-04-01), Jain et al.
patent: 5919646 (1999-07-01), Okun et al.
patent: 5932872 (1999-08-01), Price
patent: 5962520 (1999-10-01), Smith et al.
patent: 5976825 (1999-11-01), Hochman
patent: 5985549 (1999-11-01), Singer et al.
patent: 5989835 (1999-11-01), Dunlay et al.
patent: 5991028 (1999-11-01), Cabib et al.
patent: 5995143 (1999-11-01), Price et al.
patent: 6007996 (1999-12-01), McNamara et al.
patent: 6008010 (1999-12-01), Greenberger et al.
patent: 6078681 (2000-06-01), Silver
patent: 6083763 (2000-07-01), Balch
patent: 6103479 (2000-08-01), Taylor
patent: 6146830 (2000-11-01), Friend et al.
patent: 6169816 (2001-01-01), Ravkin
patent: 6222093 (2001-04-01), Marton et al.
patent: 6345115 (2002-02-01), Ramm et al.
patent: 6615141 (2003-09-01), Sabry et al.
patent: 6658143 (2003-12-01), Hansen et al.
patent: 2002/0141631 (2002-10-01), Vaisberg et al.
patent: 2002/0154798 (2002-10-01), Cong et al.
patent: 0317139 (1988-04-01), None
patent: 0468705 (1992-01-01), None
patent: 0902394 (1999-03-01), None
patent: WO 87/02802 (1987-05-01), None
patent: WO 93/21511 (1993-10-01), None
patent: WO94/11841 (1994-05-01), None
patent: WO 95/10036 (1995-04-01), None
patent: WO/9522749 (1995-08-01), None
patent: WO 96/01438 (1996-01-01), None
patent: WO 96/09605 (1996-03-01), None
patent: WO 97/20198 (1997-06-01), None
patent: WO 97/40055 (1997-10-01), None
patent: WO 97/43732 (1997-11-01), None
patent: WO 97/45730 (1997-12-01), None
patent: WO 98/05959 (1998-02-01), None
patent: WO 98/35256 (1998-08-01), None
patent: WO 98/38490 (1998-09-01), None
patent: WO 98/44333 (1998-10-01), None
patent: WO 98/45704 (1998-10-01), None
patent: WO 99/05323 (1999-02-01), None
patent: WO 99/08091 (1999-02-01), None
patent: WO 99/17116 (1999-04-01), None
patent: WO 99/39184 (1999-08-01), None
patent: WO 99/44062 (1999-09-01), None
patent: WO 99/54494 (1999-10-01), None
patent: WO 99/67739 (1999-12-01), None
patent: WO 00/03246 (2000-01-01), None
patent: WO 00/06774 (2000-02-01), None
patent: WO 00/17624 (2000-03-01), None
patent: WO 00/17643 (2000-03-01), None
patent: WO 00/17808 (2000-03-01), None
patent: WO 00/26408 (2000-05-01), None
patent: WO 00/29984 (2000-05-01), None
patent: WO 00/31534 (2000-06-01), None
patent: WO 00/33250 (2000-06-01), None
patent: WO 00/43820 (2000-07-01), None
patent: WO 00/49540 (2000-08-01), None
patent: WO 00/50872 (2000-08-01), None
patent: WO 00/60356 (2000-10-01), None
patent: WO 00/65472 (2000-11-01), None
patent: WO00/70528 (2000-11-01), None
patent: WO0135072 (2001-05-01), None
D.L. Taylor, “The new vision of light microscopy”, American Scientist 80:322-335, 1992.
K. A. Giuliano et al., “Measurement and manipulation of cytoskeletal dynamics in living cells”, Current Opinion in Cell Biology 7:4-12, 1995.
BioDx, Internet Archive Way-Back Machine, Feb. 4, 1997 From website www.biodx.com.
A. Waggoner et al., “Multiparameter Fluorescence imaging microscopy: re-agents and instruments” Human Pathology, vol. 27, No. 5, 494-502, 1996.
Benveniste et al., “Characterization of Internalization and endosome formation of epidermal growth factor in transfected NIH-3T3 cells by computerized image-intensified three-dimensional fluorescence microscopy”, The Journal of Cell Biology 109: 2105-2115, 1989.
K.L. Carey et al., “Evidence using a green fluorescent protein-glucocorticoid receptor chimera that the RAN/TC4 GTPase mediates an essential function independent of nuclear protein import”, The Journal of Cell Biology, vol. 133, No. 5, 985-996, 1996.
J. Kolega et al., “Quantitation of cytoskeletal fibers in fluorescence images: stress fiber disassembly accompanies dephosphorylation of the regulatory light chains of myosin II”, Bioimaging 1:136-150, 1993.
D.L. Farkas et al., “Multimode light microscopy and the dynamics of molecules, cells, and tissues”, Annu. Rev. Physiol. 55:785-817, 1993.
W. Böcker et al., Automated cell cycle analysis with fluorescent microscopy and image analysis, Phys. Med. Biol. 41:523-537, 1996.
R. Pepperkok et al., “System for quantitation of gene expression in single cells by computerized microimaging: Application to c-fosexpression after microinjection of anti-casein kinase II antibody”, Experimental Cell Research 204:278-285, 1993.
F. Hanakam, “Myristoylated and non-myristoylated forms of the pH sensor protein hisactophilin II: intracellular shuttling to plasma membrane and nucleus monitored in real time by a fusion with green fluorescent protein”, The EMBO Journal 15(12):2935-43, 1996.
N.B. Cole, “Golgi Dispersal during microtubule disruption: Regeneration of Golgi stacks at Peripheral Endoplasmic Reticulum Exit sites,” Molecular Biology of the Cell, vol. 7, 631-650, 1996.
B.M. Machiels Subcellualr localization of proteasomes in apoptotic lung tumor cells and persistence as compared to intermediate filaments European Journal of Cell Biology 70:250-259, 1996.
N. Yasuhara et al., “Essential Role of active nuclear transport in apoptosis” Genes to Cells 2:55-64, Jan. 1997.
BioDx, Inc., Internet archive, way back machine May 21, 1997 From website www.biodx.com.
M.V. Rogers, “Light on high -throughput screening: fluorescence-based assay technologies”, Drug Discovery Today, vol. 2, No. 4, 156-160 Apr. 1997.
W. Böcker et al., “Image Processing algorithms for the automated micronucleus assay in binucleated human lymphocytes”, Cytometry 19:283-294 (1995).
Lansing D. Taylor, U.S. Appl. No. 60/018,696, filed May 30, 1996.
Mattheakis et al., PCT Search Report for Int'l Application No. PCT/US2004/022970, Int'l Filing Date Jul. 15, 2004, dated Dec. 1, 2004.
Mattheakis et al., PCT Written Opinion for Int'l Application No. PCT/US2004/022970, Int'l Filing Date Jul. 15, 2004.
Towner et al., “Non-Invasive in Vivo Magnetic Resonance Imaging Assessment of Acute Aflatoxin B1 Hepatotoxicity in Rats”, BBA- General Subjects, Elsevier Science Publishers, NL, vol. 1475, No. 3, Jul. 26, 2000, pp. 314-320.
Sturgeon et al., &#x
Coleman Daniel A.
Vaisberg Eugeni A.
Beyer & Weaver, LLP
Cytokinetics Inc.
Tabatabai Abolfazl
Wu Jingge
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