Plates for assay of sugar chaindirected antibody and production

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is organic

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436822, 436827, G01N 33544

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active

048513571

ABSTRACT:
The invention relates to an assay plate for a sugar chain-directed antibody on which a mucin-type glycopeptide is fixed in a solid phase in the presence of an alkylenedialdehyde and a basic poly-peptide or basic synthetic polymer. The invention also relates to an assay plate wherein a serum-type glycopeptide is fixed in a solid phase by acylation with a higher fatty acid, which makes an assay fo sugar chain-directed antibody possible. Moreover, the invention makes the preparation of a hybridoma producing sugar chain-directed antibody possible, and relates to a process for the production thereof.

REFERENCES:
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patent: 4075194 (1978-02-01), Sela
patent: 4407965 (1983-10-01), Yanaihara
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patent: 4493793 (1985-01-01), Chu
patent: 4571382 (1986-02-01), Adachi
patent: 4607009 (1986-08-01), Steplewski
patent: 4623629 (1986-11-01), Kerschensteiner
patent: 4675287 (1987-06-01), Reisfeld
Liebert et al., "Journal of Immunological Methods", 85, pp. 97-104 (1985).
Metzgar et al., "Proc. Natl. Acad. Sci. U.S.A.", vol. 81, pp. 5242-5246, Aug. 1984.
Rice et al., Chemical Abstracts 99:51537g, p. 381 (1983).
Perrin et al., Chemical Abstracts 105:77142s, (1986).
Delpech et al., Chemical Abstracts 103:119095v, p. 349 (1985).

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