Platelet immunoglobulin bead suspension and flow cytometry

Details Platelet immunoglobulin bead suspension and flow cytometry Platelet immunoglobulin bead suspension and flow cytometry

2005-08-23

2005-08-23

Chin, Chris (Department: 1641)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

C435S006120, C435S007100, C435S007200, C435S007930, C435S007940, C435S007950, C435S040510, C435S173400, C435S967000, C435S973000, C436S010000, C436S063000, C436S501000, C436S506000, C436S507000, C436S509000, C436S513000, C436S518000, C436S519000, C436S523000, C436S524000, C436S527000, C436S528000, C436S531000, C436S533000, C436S534000, C436S536000, C436S538000, C436S546000, C436S805000, C436S811000

Reexamination Certificate

active

06933106

ABSTRACT:
Immunoassay methods and apparatus are provided which utilize flow cytometry, coated latex microspheres, and fluorochrome labeled antibodies, to simultaneously detect the presence and amount of one or more analytes in a sample. By combining FALS and fluorescence, it is practical to use beads of several different sizes, colors or shapes, each bead coated with a different analyte, for the simultaneous detection of one or more analytes and of cell components such as platelets in a sample.

REFERENCES:
patent: 6268222 (2001-07-01), Chandler et al.
patent: 6280618 (2001-08-01), Watkins et al.
patent: 2003/0008410 (2003-01-01), Hechinger
patent: 2003/0032068 (2003-02-01), Hechinger
Greenwalt et al. An enzyme linked antiglobulin test to quantify nanogram quantities of IgG on polystyrene microspheres. Vox Sang. 63 (4) pp. 272-275 (1992).
Rosenfeld et al., Detection of platelet alloantibodies by flow cytometry. Characterization and Clinical Significance. Am J Clin. Path. 85(2), pp. 207-212 (1986).

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