Platelet blockade assay

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435975, 436 69, 436527, 436533, G01N 33546, G01N 33552

Patent

active

057631999

ABSTRACT:
The present invention is an assay for determining glycoprotein IIb/IIIa receptor blockade in whole blood. Agglutinization of small polymeric beads coated with a glycoprotein IIb/IIIa ligand such as fibrinogen results when the beads are contacted with whole blood containing platelets with glycoprotein IIb/IIIa receptors that are not blocked. Failure to agglutinate indicates that blockade of the GPIIb/IIIa receptors has been achieved. In a preferred embodiment, the addition of a thrombin receptor activator results in an assay that is rapid and convenient enough to be performed at bedside and that results in agglutination of the small polymeric beads within a convenient, known period of time if the glycoprotein IIb/IIIa receptors are not blocked.

REFERENCES:
patent: 5114842 (1992-05-01), Plow et al.
patent: 5196309 (1993-03-01), Ginsberg
patent: 5246832 (1993-09-01), Michelson et al.
patent: 5266462 (1993-11-01), Hemker et al.
patent: 5275812 (1994-01-01), Gold et al.
patent: 5284751 (1994-02-01), Frelinger, III et al.
patent: 5306632 (1994-04-01), Anderson et al.
patent: 5427913 (1995-06-01), Shaw et al.
patent: 5455288 (1995-10-01), Coller et al.
patent: 5523238 (1996-06-01), Varon et al.
Beer et al., "Immobilized Arg-Gly-Asp (RGD) Peptides of Varying Lenghts as tructural Probes of the Platelet Glycoprotein IIb/lla Receptor", Blood, 79:117-128 (1992).
Vu et al., "Molecular Cloning of a Functional Thrombin Receptor Reveals a Novel Proteolytic Mechanism of Receptor Activation", Cell, 64:1057-1068 (1991).
Coller et al., "Collagen-Platelet Interactions: Evidence for a Direct Interaction of Collagen With Platelet GPla/lla and an Indirect Interaction With Platelet GPIIb/IIIa Mediated by Adhesive Proteins", Blood, 74:182-192 (1989).
Charo et al., "Inhibition of Fibrinogen Binding to GPIIb-IIIa by a GP IIIa Peptide", Biol. Chem., 266:1415-1421 (1991).
Coller et al., "Studies of Activated GPIIb/IIIa Receptors on the Luminal Surface of Adherent Platelets", J. Clin. Invest., 92:2796-2806 (1993).
Pfueller et al., "Role of Plasma Proteins in the Interaction of Human Platelets with Particles", Thrombosis Research, 12:979-990.
Kamiyama et al., "Inhibition of platelet GPIIb/IIIa binding to fibrinogen by serus factors: Studies of circulating immue complexes and platelet antibodies in patients with hemophilia, immune thrombocytopenic purpua, human immunodeficiency virus-related immune thrombocytopenic purpura, and systemic lupus erythematosus", J. Lab Clin. Med., 117:209-217 (1991).
Coller et al., "Thromboerythrocytes: In Vitro Studies of a Potential Autologous, Semi-artificial Alternative to Platelet Transfusions", J. Clin. Invest., 89:546-555 (1992).
Califf et al., "Use of a Monoclonal Antibody Directed Against the Platelet Glycoprotein IIb/IIIa Receptor in High-Risk Coronary Angioplasty", N. Engl. J. Med., 330:956-961 (1994).
Coller et al., "Monoclonal Antibodies to Platelet Glycoprotein IIb/IIla as Antithrombotic Agents", Ann. NY Acad. Sci., 614:193-213 (1991).
Lavee et al., "The effect of transfusion of fresh whole blood versus platelet concentrates after cardiac operations", J. Thoarc. Cardiovasc. Surg. 97:204-212 (1989).
Chrono-Log brochure, "Chrono-Log Aggregation Systems Improve Platelet Function Studies" (undated).
Carr, M. E. et al. "Glycoprotein IIb/IIIa Blockade Inhibits Platelet-Mediated Force Development and Reduces Gel Elastic Modulus," Thrombosis and Haemostasis, Journal of the International Society on Thrombosis and Haemostasis, 73 (3) :499-505 (1995).
Santoro, S. A. and Lawing W. J., Jr., "Competition for Related but Nonidentical Binding Sites on the Glycoprotein IIB-IIIa Complex by Peptides Derived from Platelet Adhesive Proteins," Cell, 48:867-873 (Mar. 1987).
Sheu, J.R., et al. "Triflavin, an Arg-Gly-Asp Containing Snake Venom Peptide, Inhibits Aggregation of Human Platelets Induced by Human Hepatoma Cell Line," Thrombosis Research 66:679-691 (1992).
Plow, E. F., et al. "The Effect of Arg-Gly-Asp-Containing Peptides on Fibrinogen and Von Willebrand Factor Binding to Platelets," Proc. Natl. Acad. Sci USA, 82:8057-8061 (Dec. 1985).
Coller, B. S., "Interaction of Normal, Thrombasthenic, and Bernard-Soulier Platelets with Immobilized Fibrinogen: Defective Platelet-Fibrinogen Interaction in Thrombasthenia," Blood, 55(2) :169-178 (Feb. 1980).
Coller, B. S., "A Murine Monoclonal Antibody that Completely Blocks the Binding of Fibrinogen to Platelets Produces a Thrombasthenic-like State in Normal Platelets and Binds to Glycoproteins IIb and or IIIa," J. Clin. Invest., 72:325-338, The American Society for Clinical Investigation, Inc. (1983).
Coller, B. S., et al., "Thrombin Receptor Activating Peptides: Importance of the N-Terminal Serine and its Ionization State as Judged by pH Dependence, Nuclear Magnetic Resonance Spectroscopy, and Cleavage by Aminopeptidase M," Biochemistry, 31 (47) :11713-11720 (1992).
Coller, B. S., et al., "Substituting Isoserine for Serine in the Thrombin Receptor Activation Peptide SFLLRN Confers Resistance to Aminopeptidase M-induced Cleavage and Inactivation," The Journal of Biological Chemistry, 268 (28) :20741-20743 (1993).
Peerschke, E., "Glycoprotein IIb and IIIa Retention on Fibrinogen-Coated Surfaces After Lysis of Adherent Platelets," Blood, 82 (11) :3358-3363, (Dec. 1, 1993).
Dorland's Illustrated Medical Dictionary, 26th ED., W. B. Saunders Company, 1985, p. 1364.
N.S. Kleiman et al, Jour. Amer. College Cardiol; 22, 381-389, 1993.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Platelet blockade assay does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Platelet blockade assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Platelet blockade assay will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2197751

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.