Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving blood clotting factor
Patent
1987-08-06
1988-11-29
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving blood clotting factor
435 4, 435 29, 435 2, 436 69, 424101, C12Q 156
Patent
active
047881399
ABSTRACT:
A platelet aggregation reagent for EDTA-anticoagulated blood comprises calcium chloride, sodium citrate and a platelet-aggregating agent; the calcium chloride and the sodium citrate being present in a proportion of about 1.5 micromoles: 5 micromoles to about 7.5 micromoles: 2.5 micromoles per at least about a platelet aggregating amount of the agent.
A platelet aggregation reagent container for EDTA-anticoagulated blood comprises an elongated container suitable for use in a platelet counter containing a reagent comprising about 1.5 micromoles to 7.5 micromoles of calcium chloride, about 2.5 micromoles to 5.0 micromoles of sodium citrate, and at least about a platelet aggregating amount of a platelet aggregating agent.
A method of determining platelet aggregation in EDTA-anticoagulated blood, comprises counting the number of platelets contained in an EDTA-anticoagulated blood sample to obtain an initial platelet count (IC), contacting the sample with calcium chloride, sodium citrate and a platelet-aggregating agent in proportions of at least about a platelet aggregating amount of the agent per about 450 microliters of the samples and about 1.2 micromoles to 7.5 micromoles of the calcium chloride and about 2.5 micromoles to 5.0 micromoles of the citrate, counting the number of platelets contained in the thus contacted sample at desired intervals for a period of about 1 minute to 10 minutes, selecting the maximum platelet count (MC) obtained in step (c) and calculating platelet aggregation (PA) from the equation
REFERENCES:
patent: 3989382 (1976-11-01), Kent et al.
patent: 4145185 (1979-03-01), Brinkhous et al.
Kowa-Chem. Abst., vol. 96 (1982), p. 196174j.
Rosen Sam
Streck Laboratories Inc.
LandOfFree
Platelet aggregation reagent, reagent container and method of de does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Platelet aggregation reagent, reagent container and method of de, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Platelet aggregation reagent, reagent container and method of de will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-363226