Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Reexamination Certificate
2011-02-15
2011-02-15
Kubelik, Anne (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
C800S278000, C435S320100
Reexamination Certificate
active
07888562
ABSTRACT:
The present invention relates to a plastid transformation system capable of preventing second recombination events of heterologous genes inserted into plastid genomes. More specifically, this invention relates to a plastid transformation vector carrying a promoter and a terminator derived from organisms other than tobacco. The inventive recombinant expression vector for plastid transformation is capable of mass-producing exogenous proteins on a level par with conventional vectors carrying promoter/terminator couples of tobacco origin. At the same time, it is capable of preventing second recombination events within plastids. Thus, the vector of this invention is greatly useful in producing transgenic plants since it can effect a secure introduction of heterologous genes and support normal transformation and heterologous gene expression.
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patent: 6624296 (2003-09-01), Maliga et al.
patent: WO 99/10513 (1999-03-01), None
Herz et al., “Development of novel types of plastid transformation vectors and evaluation of factors controlling expression,”Transgenic Research, 14(6):969-982, (2005).
Kavanagh et al., “Homologous Plastid DNA Transformation in Tobacco Is Mediated by Multiple Recombination Events,”Genetics, 152:1111-1122 (1999).
Staub et al., “Extrachromosomal elements in tobacco plastids,”Proceedings of National Academy of Science, 91:7468-7472 (1994).
Chung Hwa-Jee
Jeong Won Joong
Liu Jang Ryol
Min Sung Ran
Park Ju Young
Klarquist & Sparkman, LLP
Korea Research Institute of Bioscience and Biotechnology
Kubelik Anne
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