Plastid proteolytic processing enzyme that cleaves precursor pol

Details Plastid proteolytic processing enzyme that cleaves precursor pol Plastid proteolytic processing enzyme that cleaves precursor pol

1998-11-05

2000-09-12

Nashed, Nashaat T.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435212, 43525233, 4353201, 536 231, 536 232, 536 236, C12N 950, C07H 2104

Patent

active

061176664

ABSTRACT:
A new soluble plastid processing enzyme is purified and characterized. cDNAs encoding the enzyme are isolated, allowing recombinant production of the enzyme. The enzyme cleaves precursor polypeptides that are targeted to organelles such as the chloroplasts. The enzyme is useful to produce mature, active proteins in vivo or in vitro. An example of the enzyme is a chloroplast processing enzyme (CPE) with specificity for cleavage that produces functional proteins.

REFERENCES:
Abad, M. S., Clark, S. E. & Lamppa, G.K. (1989) Plant Physiol. 90, 117-124.
Abad, M.S., Oblong, J. E. & Lamppa, G. K. (1991) Plant Physiol. 96, 1220-1227.
Affholter, J. A., Fried, V. A. & Roth, R. A. (1988) Science 242, 1415-1418.
Authier et al. (Apr. 1995), Proc. Natl. Acad. Sci. USA, vol. 92, pp. 3859-3863.
Becker, A. B. & Roth, R. A. (1992) Proc. Natl. Acad. Sci. USA 89, 3835-3839.
Chen, N.-Y., Jiang, S.-Q., Klein, D. A. & Paulus, H. (1993) J. Biol. Chem. 268, 9448-9465.
Clark, S. E. & Lamppa, G. K. (1991) J. Cell. Biol. 114, 681-688.
Engler-Blum, G., Meier, M., Frank, J. & Muller, G. A. (1993 Anal. Biochem. 210, 235-244.
Finch, P. W., Wilson, R. E., Brown, K., Hickson, I. D. & Emmerson, P. T. (1986) Nuc. Acids Res. 14, 7695-7703.
Gavel, Y. & von Heijne, G. (1990) FEBS Letters 261, 455-458.
Gehm B. D., Kuo, W.-L., Perlman, R. K. & Rosner, M. R. (1993) J. Biol. Chem. 268, 7943-7948.
Guan, K. L. & Dixon, J. E. (1991) Anal. Biochem. 192, 262-267.
Hawlitschek, G., Schneider, H., Schmidt, B., Tropschug, M., Hartl, F.-U. & Neupert, W. (1988) Cell 53, 795-806.
Hirsch, S., Muckel, E., Heemeyer, F., von Heijne, G. & Soll, J. (1994) Science 266, 1989-1992.
Kuo, W. L., Gehm, B. & Rosner, M. R. (1990) Mol. Endo. 4, 1580-1591.
Lamppa, G. K. & Abad, M.S. (1987) J. Cell Biol. 105, 2641-2648.
Oblong, J. E. & Lamppa, G. K. (1992) EMBO J. 11, 4401-4409.
Paces, V., Rosenberg, L. E., Fenton, W. A. & Kalousek, F. (1993) Proc. Natl. Acad. Sci. USA 90, 5355-5358.
Perlman, R. K., Gehm, B. D., Kuo, W.-L. & Rosner, M. R. (1993) J. Biol. Chem. 268, 21538-21544.
Pierotti, A. R., Prat, A., Chesneau, V., Gaudoux, F., Leseney, A.-M., Foulon, T. & Cohen, P. (1994) Proc. Natl. Acad. Sci. USA 91, 6078-6082.
Rawlings, N.D. & Barrett, A. J. (1993) Biochem. J. 290, 205-218.
Robinson, C. & Ellis, R.J. (1984) Eur. J. Biochem. 142, 337-342.
Schnell, D. J., Kessler, F. & Blobel, G. (1994) Science 266, 1007-1012.
VanderVere, P., Bennett, T., Oblong, J. and Lamppa, G. (1995) Proc. Natl. Acad. Sci. USA 92, 7177-7181.
Witte, C., Jensen, R. E., Yaffe, M. P. & Schatz, G. (1988) EMBO J. 7, 1439-1447.
Yang, M. Geli, V., Oppliger, W., Suda, K., James, P. & Schatz, G. (1991) J. Biol. Chem. 266, 6416-6423.

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