Plasminogen activator to prevent corneal and subepithelial...

Drug – bio-affecting and body treating compositions – Enzyme or coenzyme containing – Hydrolases

Reexamination Certificate

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Reexamination Certificate

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10398677

ABSTRACT:
The present invention is directed to a method to prevent or reduce postoperative corneal subepithelial haze after excimer laser photorefractive keratectomy (PRK), laser in situ keratomileusis (LASIK) surgery. According to the method, a therapeutically effective amount of one or more plasminogen activators, most preferably urokinase (uPA), is administered topically to the surface of the affected eye at levels between 0.1 and 2,500 IU/ml, about eight to twelve times on the day of surgery, and four to eight times per day for about the next six to twelve days thereafter. The most preferred therapeutic amount is from about 0.1–1 IU uPA/ml, and also 1–10 IU/ml. Plasminogen activators that can be used in the inventions include urokinase, prourokinase, streptokinase and mutants thereof. The invention also covers topical ophthalmic compositions that include one or more plasminogen activators, most preferably uPA, to prevent or reduce postoperative corneal subepithelial haze.

REFERENCES:
patent: 6737075 (2004-05-01), Karageozian
patent: 2002/0119141 (2002-08-01), Karageozian
patent: 199 01 594 (2000-08-01), None
patent: WO 00/66139 (2000-11-01), None
Csutak et al. 2004. INvestigative Opthalmology & Visual Science, May, vol. 45, No. 5, pp. 1329-1333.
Plasminogen Activator Activity in Rabbit Tears after Excimer Laser Photorefractive Keratectomy, A.Csutak, et al., IOVS, vol. 41, No. 4, pp. S69, Mar. 15, 2000.
The Effects of Topical Corticosteroids and Plasmin Inhibitors on Refractive Outcome, Haze, and Visual Performance after Photorefractive Keratectomy, David P.S. O'Brart et al., Ophthalmology, vol. 101, No. 9, Sep. 1994, p. 1565-1574.
Plasmin-and Plasminogen- Activator Inhibitors After Excimer Laser Photorefractive Keratectomy: New Concept in Prevention of Postoperative Myopic Regression and Haze, C.P. Lohmann, MD et al., Refractive & Corneal Surgery, vol. Jul./Aug. 1993.
Results and Complications in rTPA Treatment of the Anterior Segment of the Eye, K.U.Loffler et al.,Ophthalmologe, 94, p. 50-52, Jan. 1997, with English translation.

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