Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an...
Patent
1994-05-20
1996-02-13
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an...
435215, 424 9463, C12N 1114, C12N 972, A61K 3848
Patent
active
054910820
ABSTRACT:
A plasminogen activator such as urokinase or tissue plasminogen activator is covalently bonded to a porous body composed of calcium phosphate to form a plasminogen activator-porous body complex. The tricalcium phosphate can be .alpha.-tricalcium phosphate, .beta.-tricalcium phosphate, hydroxyapatite, tetracalcium phosphate, octacalcium phosphate and mixtures thereof. In a preferred embodiment, .beta.-tricalcium phosphate which has excellent biocompatibility with the body or a mixture of .beta.-tricalcium phosphate and hydroxyapatite is used. Preferably, synthetic calcium phosphate is used is to avoid impurities contained by natural calcium phosphate. Covalent bonding is by crosslinking with glutaraldehyde, bismaleimides, dihalogenic aryls or diisocyanates, or by using cyanogen bromide, diazotization or periodic acid. The complex can be filled into a column to form a bioreactor. A granular complex can be intravascularly injected into the blood of a patient to provide thrombotic activity for a long period of time. In another embodiment, a complex of a predetermined shape can be implanted to provide thrombotic activity.
REFERENCES:
patent: 4282324 (1981-08-01), Neidleman et al.
patent: 4423149 (1983-12-01), Amon, Jr. et al.
patent: 4737411 (1988-04-01), Graves, Jr. et al.
patent: 4764466 (1988-08-01), Suyama et al.
patent: 4778471 (1988-10-01), Bajpai
patent: 5037749 (1991-08-01), Findlay
Zaborsky, O., Immobilized Enzymes, CRC Press, Cleveland, Ohio, 1973, p. 75.
Hakamatsuka Yasuharu
Irie Hiroyuki
Kawamoto Yukari
Kawamura Sukezo
Suzuki Takahiro
Director--General of Agency of Industrial Science and Technology
Naff David M.
Olympus Optical Co,. Ltd.
LandOfFree
Plasminogen activator covalently bonded to a porous body of .bet does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Plasminogen activator covalently bonded to a porous body of .bet, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Plasminogen activator covalently bonded to a porous body of .bet will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-240046