Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1975-10-08
1979-08-21
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435212, 435815, 2605026, 260544S, 260558S, 260556AR, 536 54, C07G 702, C07C10320, C07C10328
Patent
active
041652584
ABSTRACT:
A novel plasminogen activating enzyme-specific competitive inhibitor is disclosed consisting of an amino-terminated .alpha.-N-substituted aminocarboxylic acid, such as .alpha.-N-benzylsulfonyl-p-aminophenylalanine, or the acid derivatives thereof. The competitive inhibitor ligand, when covalently coupled to the surface of a water-insoluble solid support material through a spacer chain of at least 4 carbon atoms, is useful as a biospecific extracting agent for use in affinity chromatography of plasminogen activating enzymes, such as urokinase, cytokinase, and the like, from crude aqueous preparations thereof, such as mammalian urine, mammalian body tissue preparations, mammalian plasma and spent tissue culture growth medium.
REFERENCES:
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patent: 3801633 (1974-04-01), Toyoshima et al.
patent: 3834990 (1974-09-01), Werle et al.
patent: 3845097 (1974-10-01), Toyoshima et al.
patent: 3852338 (1974-12-01), Kaiser et al.
Maciag et al., Urokinase, Methods in Enzymology, vol. XXXIV 12/1974 (pp. 451-459).
Maciag et al., Purification of Urokinase by Affinity Chromatography, Enzyme Engineering, vol. 2, Plenum Press, N.Y. 10/1/74, (pp. 55-62).
Iyengar Melicote R.
Maciag Thomas
Pye E. Kendall
Weibel Michael K.
Naff David M.
University of Pennsylvania
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