Plasmids for plant transformation and method for using the same

Details Plasmids for plant transformation and method for using the same Plasmids for plant transformation and method for using the same

2006-04-18

2006-04-18

Kruse, David H (Department: 1638)

Chemistry: molecular biology and microbiology

Vector, per se

Reexamination Certificate

active

07029908

ABSTRACT:
The invention provides novel Ti-plasmid vectors forAgrobacterium-medicated plant cell transformation. Plamsids of the invention are constructed with a DNA sequence (ie., a negative selectable maker or a DNA sequence that inhibits DNA unwinding) flanking the T-DNA bordered gene of interest that reduces or eliminates the occurrence of transformants with vector read-through DNA sequence. The invention also provides methods of plant cell transformation utilizing these novel vectors.

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