Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1991-04-09
1992-11-10
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351723, 4353201, 43525231, 4352523, 536 27, 935 74, 935 29, 935 23, 935 38, C12P 2100, C12N 1563, C12N 1574, C12N 1575, C07H 1510
Patent
active
051622068
ABSTRACT:
A double-stranded DNA plasmid which includes a promoter DNA sequence that is not derived from a B. subtilis plasmid and a DNA sequence derived from a B. subtilis plasmid is useful for introducing into B. subtilis foreign DNA having a nucleic acid sequence which does for the production of a desired product. Preferably, the promoter sequence is also not derived from B. subtilis chromosomal DNA. When a foreign DNA sequence having a nucleic acid sequence coding for production of a desired product is introduced into this plasmid, another plasmid is produced which is useful for effecting expression in B. subtilis of the foreign DNA and production of the desired product. The plasmids may additionally include an inducible gene.
B. subtilis cells transformed with plasmids carrying a gene or genes coding for the production of desired products may be grown in culture and the resulting products recovered.
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Low Christopher S. F.
University of Maryland
Wax Robert A.
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