Plasmids for expression and method of producing a human manganes

Details Plasmids for expression and method of producing a human manganes Plasmids for expression and method of producing a human manganes

1992-07-10

1993-12-14

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

43525233, 4353201, C12N 902, C12N 121, C12N 1553

Patent

active

052701956

ABSTRACT:
An expression plasmid has been constructed which includes DNA encoding human manganese superoxide dismutase. Such plasmids may be introduced into host cells and the resulting cells cultured or grown under suitable conditions so as to produce enzymatically active human manganese superoxide dismutase or analogs thereof which may then be recovered. A method of producing an enzymatically active analog of human manganese superoxide dismutase by supplementing the culture media with Mn.sup.++ has been developed.

REFERENCES:
patent: 3920521 (1975-11-01), Michelson et al.
patent: 4129644 (1978-12-01), Kalopissis et al.
patent: 4425437 (1984-01-01), Riggs
patent: 4695456 (1987-09-01), Wilder
patent: 4742004 (1988-05-01), Hartman et al.
patent: 4751180 (1988-06-01), Cousens et al.
Jacob M. McCord and Irwin Fridovich, Superoxide Dismutase, J. Biol. Chem. 244(22): 6049-6055 (1969).
Judy Lieman-Hurwitz, et al., Human cytoplasmic superoxide dismustase cDNA clone: A probe for studying the molecular biology of Down syndrome, Proc. Natl. Acad. Sci. USA 79: 2808-2811 May 1982.
Stryer, L., Biochemistry, W. H. Freeman and Co., San Francisco p. 18 (1975).
Coombs, J., Dictionary of Biotechnology, Elsevier, New York p. 248 (1986).
Steinman, H. M., Superoxide Dismutase (Oberly, ed.), CRC Press, Florida pp. 11-68.
Barra, D. et al., FEBS Letters 120: 53-56 (1980).
Touati, D., J. of Bacteriology 155(3): 1078-1087 (1983).
Takeda, Y. et al., Nucleic Acids Res. 14(11): 4577-4589 (1986).
Beck, Y., et al., Nucleic Acids Res. 15(21): 9076 (1987).
Barra, D., et al., Proceedings of the Third Int'l. Conf. on Superoxide and SOD, vol. 1, pp. 336-339, Elsevier Science Publishing Co. (1983).
Barra, D., et al., Chemical Abstracts 100(19771g) (1984).
Suggs et al. (1981), Proc. Natl. Acad. Sci., vol. 78, No. 11, pp. 6613-6617.
Barra et al., J. Biol. Chem. 259:12595-601, 1984.

Affiliated with

Also associated with

Rating

Plasmids for expression and method of producing a human manganes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Plasmids for expression and method of producing a human manganes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Plasmids for expression and method of producing a human manganes will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1704599