Plasmids for chromosomal recombination of Escherichia coli

Details Plasmids for chromosomal recombination of Escherichia coli Plasmids for chromosomal recombination of Escherichia coli

2007-05-29

2007-05-29

Ketter, James (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S041000, C435S091100, C435S320100, C435S252330, C435S488000

Reexamination Certificate

active

10240712

ABSTRACT:
A temperature-sensitive plasmid which is capable of autonomous replication inEscherichia coliK-12 at 10–30° C., but, at a temperature of 33° C. or more, is incapable of autonomous replication inEscherichia coliK-12 or is distributed unhomogeneously upon the cell division ofEscherichia coliK-12, thereby not to be stably carried within cells ofEscherichia coliK-12 under said temperature, and which is incapable of autonomous replication in a microorganism belonging to the genusEscherichiaother thanEscherichia coliK-12 or is distributed unhomogeneously upon cell division of said microorganism at any temperature, thereby not to be stably carried within cells of said microorganism.

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