Plasmids and utilization thereof

Details Plasmids and utilization thereof Plasmids and utilization thereof

2004-10-29

2008-11-04

Prouty, Rebecca E. (Department: 1652)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S320100, C435S128000, C435S252200, C536S023200

Reexamination Certificate

active

07446187

ABSTRACT:
A shuttle vector is constructed by preparing a DNA region replicable in bacteria belonging to the genusRhodococcusfrom aRhodococcus-derived plasmid having the nucleotide sequence set forth as SEQ ID NO: 73 and a plasmid or its DNA fragment having the nucleotide sequence set forth as SEQ ID NO: 74, and a DNA region replicable inE. colifrom anE. coli-derived plasmid or its DNA fragment. An aminoketone asymmetric reductase gene is inserted into the shuttle vector, transformants containing the vector are created, and the aminoketone asymmetric reductase and optically active aminoalcohols are produced.

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