Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1993-04-09
1996-09-03
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 4352552, 536 235, 536 241, 530381, C12P 2106, C12N 119, C12N 1581
Patent
active
055522998
ABSTRACT:
The present invention relates to a process for producing recombinant desulphatohirudin by means of culturing microorganisms.
Concerning the codon usage of microorganisms the synthesized nucleotide sequences were joined downstream of and in reading frame with isolated promoters and signal sequences, subsequently the expression/secretion cassettes comprising the foregoing elements were inserted into plasmid DNAs allowing the cultivation of cells under selective culture conditions. E. coli, Saccharomyces and Streptomyces species were transformed with the said recombinant plasmids to biosynthesize the thrombin inhibitor desulphatohirudin HV-1 which was then isolated and identified.
The thus-produced desulphatohirudin can be used to inhibit blood coagulation.
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Albrecht K arnly
Ambrus G abor
Barab as Eva
Bark o n ee T oth Zsuzsa
Barta Istv an
Biogal Gyogyszergyar Rt.
Hendricks Keith D.
Wax Robert A.
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