Plasmidic vectors for expressing in bacillus subtilis and a meth

Details Plasmidic vectors for expressing in bacillus subtilis and a meth Plasmidic vectors for expressing in bacillus subtilis and a meth

1983-08-31

1986-12-02

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Miscellaneous

4351723, 435 91, 435 68, 935 27, 935 74, 935 73, C12N 100, C12N 1500, C12P 2100

Patent

active

046265104

ABSTRACT:
A special description is given of plasmids pSM15, pSM16 and pSM17, which are hybrid plasmids derived by coupling pUB110 and modified pE194. The described plasmids are cloning vectors in Bacillus subtilis and are characterised by a marker of resistance to Kanamycin and an EcoRI site located near the end of the ribosome recognition sequence; they can also be used to induce the coded heterologous protein from cloned DNA with sub-inhibiting doses of erythromycin.
These plasmids are prepared via the formation of plasmids pSM4, pSM5 or pSM6, which can be expressed either in B. subtilis or in E. coli.

REFERENCES:
patent: 4430434 (1984-02-01), Sanders et al.
Maniatis et al., 1982 Molecular Cloning: A Laboratory Manual Cold Spring Harbor Laboratory pp. 3 and 5.
Weisblum et al., 1979 "Plasmid Copy Number Control: Isolation and Characterization of High-Copy Number Mutants of pE194. J. Bact. v137(1), pp. 635-643.

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