Plasmid vehicle for cloning in Agrobacterium tumefaciens

Details Plasmid vehicle for cloning in Agrobacterium tumefaciens Plasmid vehicle for cloning in Agrobacterium tumefaciens

1983-06-07

1986-08-12

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Miscellaneous

536 27, 4351723, 935 29, 935 67, 935 6, C12N 100, C12N 1500, C07H 1512

Patent

active

046056270

ABSTRACT:
A cloning vector comprises a replication system derived from the pTAR plasmid and capable of stable maintenance in Agrobacterium tumefaciens. By combining the pTAR replication system with a second replication system from a host other than A. tumefaciens, shuttle vectors are obtained which allow manipulation in more than one host. The cloning vectors will usually include selectable markers having restriction enzyme sites which allow identification of recombinant molecules by insertional inactivation. By providing at least a fragment of the T-DNA region from the Ti plasmid, the subject vectors can be used to clone desired DNA fragments and transfer these fragments to the genome of a higher plant.
The strain E. coli HB101/puc0400 was deposited on June 7, 1983 at the A.T.C.C. for patent purposes and granted accession no. 39377.

REFERENCES:
Tuite et al., The EMBO Journal vol. 1, No. 5, pp. 603-608, 1982.
Kado et al., Journal of Bacteriology, vol. 145, No. 3, pp. 1365-1373, Mar. 1981.
Hicks et al., Genetic Engineering, Principles and Methods, vol. 4, Plenum Press ed. by Sellov et al., pp. 219-226, (1982).
Chilton et al., Stadler Symp. vol. 13, pp. 39-52, Univ. of Missouri (1981).
Leemans et al., Molecular Biology of Plant Tumors, ed. by Kahl et al., pp. 537-573 (1982).
Depicke et al., J Mol and Applied Genetics, vol. 1, No. 6, pp. 561-573 (1982).
Ameres et al., Recombinant DNA, Proceedings of the Third Cleveland Symposium on Macromolecules, pp. 185-197, Jun. 22-26, 1981.

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