Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1980-05-29
1982-09-14
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 68, 435317, C12N 1500
Patent
active
043496296
ABSTRACT:
A plasmid having an insertion site for a eukaryotic DNA fragment adjacent to a bacterial promoter and downstream from a prokaryotic ribosome binding site and initiator codon such that the bacterial promoter controls transcription and translation of an inserted DNA fragment is disclosed.
The production and use of such plasmids is also disclosed.
In general terms, one aspect of the present invention relates to a series of plasmid vectors having the basic characteristic of a Hind III insertion site adjacent to a tryptophan promoter and also a gene for tetracycline resistance. The plasmid vectors are by virtue of the structure thereof ideally suited to receive at the Hind III site an inserted eukaryotic DNA fragment the transcription and translation of which is under the control of the tryptophan promotor.
REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
Itakura et al., Science, 198, 1056-1063, (1977).
Bolivar et al., Gene, 2, 95-113, (1977).
Hershfield et al., J. Bacteriol., 126(1), 447-453, (1976).
Bennett et al., Proc. Natl. Acad. Sci. USA, 73(7), 2351-2355, (1976).
Collins et al., Proc. Natl. Acad. Sci. USA, 73(11), 3838-3842, (1976).
Warren et al., Nature, 274, 259-261, (1978).
Goeddel et al., Proc. Natl. Acad. Sci. USA, 76(1), 106-110, (1979).
Carey Norman H.
Emtage John S.
Hallewell Robert A.
Tacon William C. A.
G.D. Searle & Co.
Kepplinger Esther M.
Nelson Albin James
Tockman Albert
LandOfFree
Plasmid vectors, production anduse thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Plasmid vectors, production anduse thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Plasmid vectors, production anduse thereof will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2155481