Plasmid vectors for cloning and expression of a protein in a mic

Details Plasmid vectors for cloning and expression of a protein in a mic Plasmid vectors for cloning and expression of a protein in a mic

1984-12-05

1988-05-17

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4351723, 435255, 435942, 935 14, 935 28, 935 69, C12N 942, C12N 1500, C12N 500

Patent

active

047450622

ABSTRACT:
The present invention relates to a plasmid vector for cloning and expression of a protein in a microorganism, which comprises at least one structural gene which codes the synthesis of the said protein and elements which ensure the expression of the said structural gene in a microorganism, and wherein the promotion of the structural gene is ensured by the expression promotor of the .beta.-glucosidase gene in yeasts.
The present invention also relates to microorganisms transformed by the said vectors, in particular a transformed strain of S. cerevisiae, a fermentation process using the said vectors, and the enzymes prepared by the said process, in particular .beta.-glucosidase.

REFERENCES:
patent: 4243752 (1981-01-01), Skinner et al.
patent: 4275163 (1981-06-01), Gallo
patent: 4464471 (1984-08-01), Armentrout et al.
patent: 4469791 (1984-09-01), Colson et al.
patent: 4487831 (1984-12-01), Day et al.
Biological Abstracts, vol. 72, No. 8, 1981, p. 5388, No. 51899, Philadelphia, R. W. Armentrout.
Biological Abstracts, RRM, No. 21003547, Philadelphia, S. K. Picataggio.

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