Plasmid vector for expression in bacillus and used for cloning t

Details Plasmid vector for expression in bacillus and used for cloning t Plasmid vector for expression in bacillus and used for cloning t

1992-02-14

1994-08-02

Low, Christopher S. F.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4353201, 4352523, 435 691, 435 694, 4351723, 435832, 435839, 935 9, 935 13, 935 22, 935 29, 935 38, 935 56, 935 59, 935 60, 935 61, 935 74, 536 231, 536 235, 536 2351, 536 241, 536 242, C12N 121, C12N 1518, C12N 1575

Patent

active

053345312

ABSTRACT:
The description relates to a plasmid vector expression in Bacillus and used for cloning the structural gene which codes for the human growth hormone, a recombinant DNA molecule comprising plasmid vector and the structural gene of the human growth hormone and a method of preparing the hormone comprising inserting the recombinant DNA molecule into strains of Bacillus and cultivating the resulting transformed strains in a suitable culture environment and recovering the thus-synthesized hormone from the cells.

REFERENCES:
patent: 4342832 (1982-08-01), Goeddel et al.
patent: 4452775 (1984-06-01), Kent
patent: 4626510 (1986-12-01), Grandi
patent: 4760025 (1988-07-01), Estell et al.
patent: 4987078 (1991-01-01), Grandi et al.
Grandi et al. (1986) New Plasmid Expression Vector for Bacillus subtilis. Plasmid 16, 1-14.
Honjo et al. (1986) Secretion of Human Growth Hormone in Bacillus subtilis Using Prepropeptide Coding Region of Bacillus amyloliquifaciens Neutral Protease Gene. J. Biotechnol. 4, 63-71.
Gentz et al. (1985) Promoters Recognized by Escherichia coli RNA Polymerase Selected by Fuction: Highly Efficient Promoters From Bactrio T5. J. Bacteriol. 164, 70-77.
Marina Del Bue et al., Plasmid Expression Vectors For B. Subtilis, Chimicaoggi, No. 3, Mar. 1987, pp. 73-75.
Herman A. DeBoer et al., A Hybrid Promoter and Portable Shine-Dalgarno Regions of Escherichia coli, Biochemical Soc. Symposis, vol. 48, 1983, pp. 233-244.
Donna M. Williams et al., Cloning Restriction Fragments That Promote Expression of a Gene in Bacillus subtilis, Jour. of Bacter., Jun. 1981, pp. 1162-1165.
Tomoko Tokunaga et al., Expression of a Synthetic Human Growth Hormone Gene in Yeast, Gene, 39, No. 1, 1985, pp. 117-120.
Charles P. Moran, Jr. et al., Nucleotide Sequences That Signal the Initiation of Transcription and Translation in Bacillus subtilis, Mol. Gen. Gene., vol. 186, 1982, pp. 339-346.
David V. Goeddel et al., Direct Expression in Escherichia coli of a DNA Sequence Coding for Human Growth Hormone, Nature, vol. 281, Oct. 18, 1979 pp. 544-548.

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