Plasmid vector and a method for regulation of gene expression us

Details Plasmid vector and a method for regulation of gene expression us Plasmid vector and a method for regulation of gene expression us

1995-02-16

1997-07-01

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

43525232, 4353201, C12N 121, C12N 1563, C12N 1577

Patent

active

056437900

ABSTRACT:
A plasmid vector capable of replicating in a Coryneform bacterial cell bearing a base sequence (a) functioning as an promoter in a Coryneform bacterium, a base sequence (b) functioning as an operator downstream from the base sequence (a), a base sequence (c) functioning as a site for ribosome binding in a Coryneform bacterial cell, a base sequence (d) functioning as a translation initiation codon, and a gene to be expressed which is directly ligated with the base sequence (d) and bearing a gene coding for a repressor protein capable of binding to the base sequence (d) functioning as an operator.

REFERENCES:
patent: 5426050 (1995-06-01), Morinaga et al.
Santamaria et al. (1984), J. Gen. Microbiol. 130: 2237-2246.

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