Plasmid stabilization

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

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435 6, 435 691, 4352523, 43525233, 4352542, 4353201, 435325, 435440, 435455, 435463, 435466, 435476, 435483, 536 231, C12N 168, C12N 121, C12N 1585, C12N 1563

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active

059727080

ABSTRACT:
A system is described which utilizes a novel system of repressor titration for maintenance of a plasmid useful in gene therapy and production of a recombinant protein. The system utilizes a transformed host cell containing a plasmid including an operator susceptible to binding by a repressor expressed in trans, a first chromosomal gene encoding the repressor, and a second chromosomal gene that is functionally associated with an operator and essential for cell growth, wherein the plasmid is present in the cell in sufficient numbers to titrate the repressor such that the essential gene is expressed, thereby permitting cell growth.

REFERENCES:
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patent: 5015573 (1991-05-01), Yarranton et al.
patent: 5763270 (1998-06-01), Eastman et al.
Gossen, et al., "Control of gene activity in higher eukaryotic cells by prokaryotic regulatory elements", TIBS, 1993, 18, 471-475.
Lebedeva, et al., "A new T7 RNA polymerase-driven expression system induced via thermoamplification of a recombinant plasmid carrying a T7 promoter--Escherichia coli lac operator", Gene, 1994, 142, 61-66.

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