Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1992-01-30
1993-08-17
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 530350, 935 27, 935 29, 935 38, 935 40, 935 47, C12N 1573, C12N 1563, C12N 1566
Patent
active
052368289
ABSTRACT:
The plasmid of this invention contains the cII translation initiation site and a P.sub.L promoter--both are very efficient, producing large amounts of cII protein. This plasmid contains a unique ClaI restriction site in the aminoterminal portion of the cII gene suitable for insertion of foreign genes. This plasmid is an improvement over other plasmids because the inserted genes are subject to the same transcriptional and translational characteristics of the cII gene; this plasmid, therefore, produces large amounts of the protein coded by the exogenous gene.
REFERENCES:
patent: 4831120 (1989-05-01), Aviv et al.
Renaut et al., 1981, Gene, 15: 81-93.
Oppenheim et al., 1982, J. Mol. Biol. 158: 327-346.
Bolivar et al., 1977, Gene, 2: 95-113.
Lautenberger James A.
Papas Takis S.
Railey II Johnny F.
Schwartz Richard A.
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