Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2005-05-03
2005-05-03
Ketter, James (Department: 1636)
Chemistry: molecular biology and microbiology
Vector, per se
C435S252300, C435S476000
Reexamination Certificate
active
06887702
ABSTRACT:
The present invention relates to a new plasmid originated from (Bifidobacterium) a recombinant expression vector and transformation method using the same. More particularly, the present invention relates to a plasmid pMG1 having nucleotide sequence represented by SEQ.ID.NO.1; (Bifidobacterium longum) MG1 including the plasmid pMG1; and a shuttle vector which can be replicated in both (Bifidobacterium) and (E. coli), and comprises (Mob) gene having nucleotide sequence represented by SEQ.ID.NO.2. (Rep) gene having nucleotide sequence represented by SEQ.ID.NO.3 and a selection marker. The shuttle vector and the promoter of the present invention can be used for expressing target gene without additional purification process. The protein expressed from the target gene in (Bifidobacterium) can be added to food, therefore, the protein can be used for preparing food additives or oral vaccine, Furthermore, the potential and the possibilty of probiotics using (Bifidobacterium) can promoted through the development of the shuttle vector.
REFERENCES:
Missich et al., “Transformation ofBifidobacterium longumwith pRM2, a ConstructedEscherichia coli—B. longumShuttle Vector”, Plasmid, 32, 208-211 (1994).*
Park et al., “Sequence analysis of plasmid pKJ50 fromBifidobacterium longum”, Microbiology 145:585-592 (1999).*
Genbank Accession No. NC_002635 (Ji, G.E., Park, M.S., and Shin, D.W.) Jan. 2, 2001.
Joon- Seok Song et al., “Characterization of the Replication Region ofEnterococcus faecalisPlasmid p703/5,” J. Microbiol. Biotechnol., 1999, (9)1, 91-97.
Sambrook et al., Molecular Cloning, Plasmid Vectors p. 1.13, Second Edition.
Park et al., “Sequence Analysis of Plasmid pK150 fromBifidobacterium Longum,” Microbiology, vol. 145, pp. 585-592, 1999.
Ji Geun-Eog
Kang Yun-Hee
Park Myeong-Soo
Seo Jung-Min
Bifido Co., Ltd.
Foley & Lardner LLP
Ketter James
Vogel Nancy T.
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