Plasmid, method for construction of the same, microorganisms car

Details Plasmid, method for construction of the same, microorganisms car Plasmid, method for construction of the same, microorganisms car

1987-03-30

1990-10-09

Teskin, Robin L.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4351723, 435320, 435207, 935 73, 935 48, C12N 1500, C12N 120

Patent

active

049620550

ABSTRACT:
A novel plasmid pEAP2, which was constructed from Bacillus sp. 170 chromosomal DNA carrying the gene for extracellular production of penicillinase and a vector plasmid pMB9. A novel microorganism, Escherichia coli HB101(pEAP2) carrying the plasmid pEAP2 and being capable of extracellular production of penicillinase. The method for cultivation of the microorganism is characterized by culturing it in the medium containing NaCl (or KCl) for 16-48 hours. According to this invention, useful high-molecular substances can be produced in a high yield.

REFERENCES:
patent: 4411994 (1983-10-01), Gilbert et al.
Imanaka et al., T. Bact, vol. 147 (3) Sep. 1981, pp. 776-786, "Cloning of the Genes for Penicillinase, penP and penI, of Bacillus licheniformis in Some Vector Plasmids and Their Expression in Escherichia coli, Bacillus subtilis, and Bacillus licheniformis".
Gray et al., T Bact, vol. 145 (1) Jan. 1981, pp. 422-428, "Molecular Cloning and Expression of Bacillus licheniformis .beta.-Lactamase Gene in Escherichia coli and Bacillus subtilis".
Sunaga et al, Agric Biol Chem. vol. 40, pp. 1363-1367, 1976 "Production of Penicillinase by an Alkalophilic Bacillus".

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