Plasmid, method for construction of the same, microorganism carr

Details Plasmid, method for construction of the same, microorganism carr Plasmid, method for construction of the same, microorganism carr

1984-03-19

1986-11-25

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435317, 435 91, 435253, 435200, 935 14, 935 29, 935 48, 935 56, 935 73, C12N 1500, C12N 100, C12N 120, C12P 1934

Patent

active

046249222

ABSTRACT:
A novel plasmid pCX311, which was constructed from Bacillus sp. C125 chromosomal DNA carrying the gene for extracellular production of xylanase and a vector plasmid pBR322. A novel microorganism, Escherichia coli HB101 (pCX311) carrying the plasmid pCX311 and being capable of extracellular production of xylanase. Method for culturing the microorganism characterized by cultivation of it in the medium containing NaCl (or KCl) and bran, or NaCl (or KCl) and xylan for 12-48 hours. According to this invention, useful high-molecular substances can be produced in a high yield.

REFERENCES:
Horikoshi et al, 1973, "Xylanase Produced by Alkalophilic Bacillus, No. C-59-2", Agr. Biol. Chem., vol. 37(9), 2097-2103.
Panbangred et al, 1983, "Molecular Cloning of the Genes for Xylan Degradation of Bacillus pumilus . . . " Mol Gen Genet, vol. 192, pp. 335-341.
Panbangred et al, 1984, "Isolation of Two .beta.-Xylosidase Genes of Bacillus pumilus and Comparison of Their Gene Prod.", E.J. Bioch , vol. 138, pp. 267-273.
Honda et al, 1985, "Molecular Cloning and Expression of the Xylanase Gene of Alkalophilic Bacillus sp . . . ", J.Bact, vol. 161(2), pp. 784-785.
Bernier, Jr. et al, 1983, "Molecular Cloning of a Bacillus subtilis Xylanase Gene in E. coli", Gene, vol. 26, pp. 59-65.

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