Plasmid maintenance

Details Plasmid maintenance Plasmid maintenance

2004-11-22

2009-11-03

McGarry, Sean R (Department: 1635)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S480000, C435S481000, C435S486000, C435S488000, C435S091400

Reexamination Certificate

active

07611883

ABSTRACT:
The invention relates to a system for stable maintenance of a plasmid, to host cells for use in this system and to methods of using the system to obtain a plasmid useful in medical applications. In particular, the invention provides transformed host cell containing: i) a chromosomal gene which inhibits cell growth; and ii) a plasmid encoding an antisense sequence, wherein the antisense sequence encoded by the plasmid inhibits the action of the chromosomal gene, thereby permitting cell growth and a method for stable maintenance of a plasmid in a host cell in vivo.

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