Plasmid for the overproduction of bacteriophage T3 RNA polymeras

Details Plasmid for the overproduction of bacteriophage T3 RNA polymeras Plasmid for the overproduction of bacteriophage T3 RNA polymeras

1986-04-01

1991-08-06

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

4353201, 435 6, 4351723, 435194, 4352351, 935 31, 935 17, C12N 701, C12N 912, C12N 1500, C12N 1510

Patent

active

050377450

ABSTRACT:
A dual phage RNA polymerase promoter having a polylinker with at opposite ends, a T3 phage RNA polymerase promoter and a phage RNA polymerase promoter, like T7; the promoters are linked to the polylinker in opposite orientation. A recombinant DNA vector containing a T3 phage RNA polymerase promoter and a different phage RNA polymerase promoter; the promoters are linked to a polylinker sequence in opposite orientation. A kit including a T3 promoter and containing appropriate components for synthesizing RNA transcripts that are complementary to either strands of a cloned DNA sequence, and for other applications. Gene coding for T3 RNA polymerase, a vector containing the gene and transformed microorganisms.

REFERENCES:
patent: 4766072 (1988-08-01), Jendrisak et al.
Bluescribe Advertisement, 1985.
BRL Catalogue, 1985.
McGraw, N. et al, Nuc Acid Res, vol. 13, pp. 6753-6766, Sep. 1985.
Melton, D. et al, Nuc. Acids Res., vol. 12, pp. 7035-7056, 1984.
Axelrod, V. et al, Biochemistry, vol. 24, pp. 5716-5723, Oct. 1985.
Pharmacia Advertisement in Nature, vol. 317, Oct. 1985.
Adhya, S. et al, Proc. Natl. Acad. Sci., vol. 78, pp. 147-151, 1981.
Bailey, J. et al., Proc. Natl. Acad. Sci., vol. 80, pp. 2814-2818, May, 1983.

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