Plasmid for production of CRM protein and diphtheria toxin

Details Plasmid for production of CRM protein and diphtheria toxin Plasmid for production of CRM protein and diphtheria toxin

1995-05-25

1997-03-25

Elliott, George C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

43525232, 4353201, C12P 2106, C12N 120, C12N 1503, C12N 1563

Patent

active

056143826

ABSTRACT:
The invention pertains to a novel method and plasmid system for producing abundant quantities of CRM197 protein, diphtheria toxin or other CRM proteins related to diphtheria toxin, as well as to microorganisms transformed with the novel plasmid. A particularly preferred DNA plasmid, designated pPX 3511, that combines the gene for CRM197 from the nontoxigenic betaphage and the plasmid pNG2-22 is described. The novel plasmid system is capable of transforming strains of Corynebacterium diphtheriae into strains which are capable of expressing high levels of the CRM197 protein without the use of multiple lysogens. The invention provides an elegant means for increasing protein production without having to manipulate the expression vector, such as by increasing the promoter strength, or removing the promoter from iron regulation.

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