Plasmid for foreign gene expression in B. subtilis

Details Plasmid for foreign gene expression in B. subtilis Plasmid for foreign gene expression in B. subtilis

1983-03-07

1986-04-29

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435317, 4351723, 935 29, 935 38, 935 60, C12N 120, C12N 100, C12N 1500

Patent

active

045857393

ABSTRACT:
The invention provides a mutant plasmid useful for introducing into B. subtilis foreign DNA, the nucleic acid sequence of which codes for production of a desired product. The plasmid is constructed from (1) a segment of B. pumllus DNA containing a chloramphenicol acetyltransferase gene plus an EcoRI* promoter fragment cloned from phage SPO2 DNA and a (2) portion of a B. subtilis plasmid, pUB110. The plasmid gives enhanced expression of genes coding for certain products, such as dihydrofolate reductase.

REFERENCES:
Wallace et al., Science, vol. 209, pp. 1396-1400, Sep. 19, 1980.
Smith et al., Genetic Engineering, Principles and Methods, vol. 3, edited by Setlow et al., Plenum Press, pp. 1-32 (1981).
Lovett et al., J. Bacteriol. 127, 817-828 (1976).
Ehrlich, Proc. Natl. Acad. Sci. USA 74, 1680-1682 (1977).
Keggins et al., Proc. Natl. Acad. Sci. USA 75, 1423-1427.
Gryczan et al., Proc. Natl. Acad. Sci. USA 75, 1428-1432 (1978).
Goldbard et al., Nature 293, 309-311 (1981).
Williams et al., Gene 16, 199-206 (1981).

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